This study aimed to evaluate different recovery techniques of oocytes and the duration time between ovary collection and processing in the laboratory on the quality and quantity of Simmental cross bovine oocytes in vitro maturation. A total of 75 bovine ovaries were divided into three groups. Experiment-1; the storage duration of ovaries were 6, 9 and 12 h. Experiment -2; the techniques for oocytes recovery were slicing, aspiration and slicing + aspiration. The ovaries were collected from slaughterhouse and kept in saline solution during transportation to laboratory. A total of 136 oocytes were used for experiment-1, and 246 oocytes for experiment-2. This study found that the number and quality of oocytes were significantly different (P0.05) on oocytes quality. The average of oocytes of groups slicing, aspiration and slicing plus aspiration were 14.66±2.09; 9.46±2.99 and 16.40±6.86, respectively. Both experiment was significant effect (P