Bioinformatics Analysis of Key Genes and Pathways of Cervical Cancer
Open Access
- 1 December 2020
- journal article
- research article
- Published by Taylor & Francis Ltd in OncoTargets and Therapy
- Vol. ume 13, 13275-13283
- https://doi.org/10.2147/ott.s281533
Abstract
Background and Objective: Globally, cervical cancer (CC) is the fourth most common cancer affecting women. Although effective screening reduces its incidence, it remains one of the most serious cancers threatening the health of women. Therefore, the purpose of this study is to find new genes that can be used as potential biomarkers for the prognosis of CC. Methods and Results: After downloading three datasets such as GSE6791, GSE63678, and GSE63514 from the Gene Expression Omnibus (GEO), we combined the expression matrixes and analyzed them to obtain the differential expressed genes (DEGs). Next, using the STRING website, we performed the protein interaction network analysis. Subsequently, hub genes were screened using the R and Cytoscape software. Then, the expression difference and survival analyses of the hub genes were confirmed using GIPIA. Here, we established that the KNTC1 gene was correlated to the overall survival prognosis of CC. Besides, the expression of the KNTC1 gene in the GSE63514 dataset was significantly different from that of the normal cervix, cervical pre-cancerous lesions, and CC. Consequently, immunohistochemistry analysis showed that the results have a definite diagnostic value. Conclusion: The KNTC1 gene could be linked with the pathophysiology of CC and maybe one of the early diagnostic markers for the diagnosis of cervical pre-cancerous lesions.Keywords
This publication has 43 references indexed in Scilit:
- The effectiveness of conization treatment for post-menopausal women with high-grade cervical intraepithelial neoplasiaExperimental and Therapeutic Medicine, 2012
- clusterProfiler: an R Package for Comparing Biological Themes Among Gene ClustersOMICS: A Journal of Integrative Biology, 2012
- Array-based gene expression, CGH and tissue data defines a 12q24 gain in neuroblastic tumors with prognostic implicationBMC Cancer, 2010
- Locus Reference Genomic sequences: an improved basis for describing human DNA variantsGenome Medicine, 2010
- Modulations of cell cycle checkpoints during HCV associated diseaseBMC Infectious Diseases, 2009
- Fundamental Differences in Cell Cycle Deregulation in Human Papillomavirus–Positive and Human Papillomavirus–Negative Head/Neck and Cervical CancersCancer Research, 2007
- Aurora-kinase inhibitors as anticancer agentsNature Reviews Cancer, 2004
- Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction NetworksGenome Research, 2003
- Determinants of cervical cancer rates in developing countriesInternational Journal of Cancer, 2002
- Papillomaviruses and cancer: from basic studies to clinical applicationNature Reviews Cancer, 2002