Engineered Orange Ectopically Expressing the Arabidopsis β-Caryophyllene Synthase Is Not Attractive to Diaphorina citri, the Vector of the Bacterial Pathogen Associated to Huanglongbing
Open Access
- 2 March 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Plant Science
Abstract
Huanglongbing (HLB) is a destructive disease, associated with psyllid-transmitted phloem-restricted pathogenic bacteria, which is seriously endangering citriculture worldwide. It affects all citrus species and cultivars regardless of the rootstock used, and despite intensive research in the last decades, there is no effective cure to control either the bacterial species (Candidatus Liberibacter spp.) or their insect vectors (Diaphorina citri and Trioza erytreae). Currently, the best attempts to manage HLB are based on three approaches: (i) reducing the psyllid population by intensive insecticide treatments; (ii) reducing inoculum sources by removing infected trees, and (iii) using nursery-certified healthy plants for replanting. The economic losses caused by HLB (decreased fruit quality, reduced yield, and tree destruction) and the huge environmental costs of disease management seriously threaten the sustainability of the citrus industry in affected regions. Here, we have generated genetically modified sweet orange lines to constitutively emit (E)-β-caryophyllene, a sesquiterpene repellent to D. citri, the main HLB psyllid vector. We demonstrate that this alteration in volatile emission affects behavioral responses of the psyllid in olfactometric and no-choice assays, making them repellent/less attractant to the HLB vector, opening a new alternative for possible HLB control in the field.Keywords
Funding Information
- Fundo de Defesa da Citricultura
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- H2020 European Research Council
This publication has 88 references indexed in Scilit:
- Citrus Leaf Volatiles as Affected by Developmental Stage and Genetic TypeInternational Journal of Molecular Sciences, 2013
- Vertical T-maze Choice Assay for Arthropod Response to OdorantsJournal of Visualized Experiments, 2013
- Inconsequential effect of nutritional treatments on huanglongbing control, fruit quality, bacterial titer and disease progressCrop Protection, 2012
- Induced Release of a Plant-Defense Volatile ‘Deceptively’ Attracts Insect Vectors to Plants Infected with a Bacterial PathogenPLoS Pathogens, 2012
- Reference Genes for Accurate Transcript Normalization in Citrus Genotypes under Different Experimental ConditionsPLOS ONE, 2012
- Perception of plant volatile blends by herbivorous insects – Finding the right mixPhytochemistry, 2011
- Effects of huanglongbing on fruit quality of sweet orange cultivars in BrazilEuropean Journal of Plant Pathology, 2009
- A Maize (E)-β-Caryophyllene Synthase Implicated in Indirect Defense Responses against Herbivores Is Not Expressed in Most American Maize VarietiesTHE PLANT CELL ONLINE, 2008
- Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbingJournal of Microbiological Methods, 2006
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001