The Property-Based Practical Applications and Solutions of Genetically Encoded Acetylcholine and Monoamine Sensors
Open Access
- 24 February 2021
- journal article
- research article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 41 (11), 2318-2328
- https://doi.org/10.1523/jneurosci.1062-19.2020
Abstract
Neuromodulatory communication among various neurons and non-neuronal cells mediates myriad physiological and pathologic processes, yet defining regulatory and functional features of neuromodulatory transmission remains challenging because of limitations of available monitoring tools. Recently developed genetically encoded neuromodulatory transmitter sensors, when combined with superresolution and/or deconvolution microscopy, allow the first visualization of neuromodulatory transmission with nanoscale or microscale spatiotemporal resolution. In vitro and in vivo experiments have validated several high-performing sensors to have the qualities necessary for demarcating fundamental synaptic properties of neuromodulatory transmission, and initial analysis has unveiled unexpected fine control and precision of neuromodulation. These new findings underscore the importance of synaptic dynamics in synapse-, subcellular-, and circuit-specific neuromodulation, as well as the prospect of genetically encoded transmitter sensors in expanding our knowledge of various behaviors and diseases, including Alzheimer's disease, sleeping disorders, tumorigenesis, and many others.Funding Information
- HHS | NIH | National Institute of Neurological Disorders and Stroke (NS104670)
This publication has 127 references indexed in Scilit:
- The organization of two new cortical interneuronal circuitsNature Neuroscience, 2013
- Noradrenergic modulation of wakefulness/arousalSleep Medicine Reviews, 2012
- Extrasynaptic GABAA Receptors: Their Function in the CNS and Implications for DiseaseNeuron, 2012
- Characterization of the proteome, diseases and evolution of the human postsynaptic densityNature Neuroscience, 2010
- Reassessment of the structural basis of the ascending arousal systemJournal of Comparative Neurology, 2010
- Tuning arousal with optogenetic modulation of locus coeruleus neuronsNature Neuroscience, 2010
- Recent Advances in Lentiviral Vector Development and ApplicationsMolecular Therapy, 2010
- Acetylcholine beyond neurons: the non‐neuronal cholinergic system in humansBritish Journal of Pharmacology, 2008
- Monitoring Rapid Chemical Communication in the BrainChemical Reviews, 2008
- The optimal height of the synaptic cleftProceedings of the National Academy of Sciences of the United States of America, 2007