Circulating tumour cell liquid biopsy in selecting therapy for recurrent cutaneous melanoma with locoregional pelvic metastases: a pilot study
Open Access
- 24 March 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Research Notes
- Vol. 13 (1), 1-8
- https://doi.org/10.1186/s13104-020-05021-5
Abstract
Circulating tumour cells (CTCs) from liquid biopsies provide an exceptional opportunity to obtain real-time tumour information and are under current investigation in several cancers, including cutaneous melanoma, but face significant drawbacks in terms of non-standardised methodology, low viable cell numbers and accuracy of CTC identification. In this pilot study, we report that chemosensitivity assays using liquid biopsy-derived metastatic melanoma (MM) CTCs, from 7 patients with stage IIIC, BRAF wild-type metastatic melanomas, localized exclusively to the pelvic region, un-eligible for immunotherapy and treated with melphalan hypoxic pelvic perfusion (HPP), is both feasible and useful in predicting response to therapy. Viable MM CTCs (> 5 cells/ml for all 7 blood samples), enriched by transient culture, were characterised in flow cytometry-based Annexin V-PE assays for chemosensitivity to several drugs. Using melphalan as a standard, chemosensitivity cut-off values of > 60% cell death, were predictive of patient RECIST 1.1 response to melphalan HPP therapy, associated with calculated 100% sensitivity, 66.67% specificity, 33.33% positive predictive, 100% negative predictive, and 71.43% accuracy values. We propose that the methodology in this study is both feasible and has potential value in predicting response to therapy, setting the stage for a larger study. Trial registration Clinical Trials.gov Identifier NCT01920516; date of trial registration: August 6, 2013Keywords
This publication has 33 references indexed in Scilit:
- Phase 2 Study of Intralesional PV-10 in Refractory Metastatic MelanomaAnnals of Surgical Oncology, 2014
- The nature and management of metastatic melanoma after progression on BRAF inhibitors: Effects of extended BRAF inhibitionCancer, 2014
- Survival, Durable Tumor Remission, and Long-Term Safety in Patients With Advanced Melanoma Receiving NivolumabJournal of Clinical Oncology, 2014
- Anvirzel™ in combination with cisplatin in breast, colon, lung, prostate, melanoma and pancreatic cancer cell linesBMC Pharmacology and Toxicology, 2013
- Melanoma metastases in the abdomen and pelvis: Frequency and patterns of spreadWorld Journal of Radiology, 2013
- Electrochemotherapy for disseminated superficial metastases from malignant melanomaBritish Journal of Surgery, 2012
- Role of Radiation Therapy in Cutaneous MelanomaThe Cancer Journal, 2012
- The role and interpretation of pilot studies in clinical researchJournal of Psychiatric Research, 2011
- Regional Treatment Strategies for In-Transit Melanoma MetastasisSurgical Oncology Clinics of North America, 2011
- New response evaluation criteria in solid tumours: Revised RECIST guideline (version 1.1)European Journal of Cancer, 2009