Effect of ciRS-7 expression on clear cell renal cell carcinoma progression
Open Access
- 2 June 2020
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Chinese Medical Journal
- Vol. 133 (17), 2084-2089
- https://doi.org/10.1097/cm9.0000000000000867
Abstract
Background Circular RNA ciRS-7 has been reported to be involved in the progression of various cancers. However, ciRS-7 expression and its role in clear cell renal cell carcinoma (ccRCC) progression remains unclear. This study aimed to investigate the effect of ciRS-7 expression on ccRCC and the related signaling pathway. Methods ciRS-7 expression was analyzed using quantitative reverse transcription polymerase chain reaction in 87 pairs of ccRCC and matched adjacent normal tissues. The role of ciRS-7 in ccRCC cell proliferation and invasion was determined using the cell counting kit-8 and invasion assays, respectively. Potential mechanisms underlying the role of ciRS-7 in promoting ccRCC progression were explored by Western blotting. The relationship between the expression of ciRS-7 and features of ccRCC was analyzed by the Chi-square test and progression-free survival was determined using a Kaplan-Meier plot. Results ciRS-7 was overexpressed in ccRCC tissues compared with that in matched adjacent normal tissues. In addition, ciRS-7 up-regulation was closely associated with tumor diameter (P= 0.050), clinical stage (P = 0.009), and distant metastasis (P = 0.007). ciRS-7 knockdown in 786O and 769P cells markedly inhibited their proliferative and invasive abilities. In addition, ciRS-7 inhibition reduced phosphorylated epidermal growth factor receptor (p-EGFR) and phosphorylated serine/threonine kinase (p-Akt) levels. Conclusions ciRS-7 up-regulation could promote ccRCC cell proliferation and invasion, which may be related with the EGFR/Akt signaling pathway. ciRS-7 might be a potential ccRCC therapeutic target.Keywords
This publication has 24 references indexed in Scilit:
- The biogenesis and emerging roles of circular RNAsNature Reviews Molecular Cell Biology, 2016
- SnapShot: Renal Cell CarcinomaCell, 2015
- The emerging landscape of circular RNA ciRS-7 in cancer (Review)Oncology Reports, 2015
- The expression and role of tyrosine kinase ETK/BMX in renal cell carcinomaJournal of Experimental & Clinical Cancer Research, 2014
- Targeted therapy in metastatic renal carcinomaCancer Letters, 2014
- Circular RNA and miR-7 in CancerCancer Research, 2013
- Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell TypesPLOS ONE, 2012
- Obesity, Cholesterol, and Clear-Cell Renal Cell Carcinoma (RCC)Advances in Cancer Research, 2010
- Mutations of the VHL gene in sporadic renal cell carcinoma: Definition of a risk factor for VHL patients to develop an RCCHuman Mutation, 1999
- Some yeast mitochondrial RNAs are circularCell, 1980