Serotonin involvement in okadaic acid-induced diarrhoea in vivo
Open Access
- 20 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archives of Toxicology
- Vol. 95 (8), 2797-2813
- https://doi.org/10.1007/s00204-021-03095-z
Abstract
The consumption of contaminated shellfish with okadaic acid (OA) group of toxins leads to diarrhoeic shellfish poisoning (DSP) characterized by a set of symptoms including nausea, vomiting and diarrhoea. These phycotoxins are Ser/Thr phosphatase inhibitors, which produce hyperphosphorylation in cellular proteins. However, this inhibition does not fully explain the symptomatology reported and other targets could be relevant to the toxicity. Previous studies have indicated a feasible involvement of the nervous system. We performed a set of in vivo approaches to elucidate whether neuropeptide Y (NPY), Peptide YY (PYY) or serotonin (5-HT) was implicated in the early OA-induced diarrhoea. Fasted Swiss female mice were administered NPY, PYY(3–36) or cyproheptadine intraperitoneal prior to oral OA treatment (250 µg/kg). A non-significant delay in diarrhoea onset was observed for NPY (107 µg/kg) and PYY(3–36) (1 mg/kg) pre-treatment. On the contrary, the serotonin antagonist cyproheptadine was able to block (10 mg/kg) or delay (0.1 and 1 mg/kg) diarrhoea onset suggesting a role of 5-HT. This is the first report of the possible involvement of serotonin in OA-induced poisoning.Keywords
Funding Information
- Ministerio de Economía, Industria y Competitividad, Gobierno de España (AGL2016-78728-R (AEI/FEDER, UE), IISCIII/PI19/001248)
- Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia (2017 GRC GI-1682 (ED431C 2017/01)
- European Commission (Interreg AlertoxNet EAPA-317-2016, Interreg Agritox EAPA-998-2018)
- Horizon 2020 (778069-EMERTOX)
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