Rapid Determination of Cephalexin in Animal-Derived Food by an Indirect Competitive Enzyme-Linked Immunosorbent Assay
- 11 May 2022
- journal article
- research article
- Published by Taylor & Francis Ltd in Analytical Letters
- Vol. 55 (17), 2803-2816
- https://doi.org/10.1080/00032719.2022.2072857
Abstract
Cephalexin (CFE) residues in animal-derived food and its deleterious effects on human health has attracted extensive attention. In this study, a specific anti-cephalexin monoclonal antibody (mAb) was used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) with a half-maximal inhibitory concentration (IC50) of 1.83 µg/L that was suitable for the determination of cephalexin in animal-derived foods. The limit of detection (LOD) and limit of quantitation (LOQ) for cephalexin were from 0.44 to 0.58 µg/kg and 0.57 to 0.67 µg/kg, respectively. The recovery was from 90.1 to 103.6% with coefficients of variation (CV) less than 10.0%. In addition, the established ELISA method and high-performance liquid chromatography – mass spectrometry (HPLC-MS/MS) were used to analyze the samples with good correlation between the results. The results show that the established ic-ELISA is suitable for the determination of cephalexin in animal-derived food.Keywords
Funding Information
- the Key Research and Development Programof Hubei Province
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