Methodological Approach of the Iron and Muscular Damage: Female Metabolism and Menstrual Cycle during Exercise Project (IronFEMME Study)
Open Access
- 16 January 2021
- journal article
- research article
- Published by MDPI AG in International Journal of Environmental Research and Public Health
- Vol. 18 (2), 735
- https://doi.org/10.3390/ijerph18020735
Abstract
Background: The increase in exercise levels in the last few years among professional and recreational female athletes has led to an increased scientific interest about sports health and performance in the female athlete population. The purpose of the IronFEMME Study described in this protocol article is to determine the influence of different hormonal profiles on iron metabolism in response to endurance exercise, and the main markers of muscle damage in response to resistance exercise; both in eumenorrheic, oral contraceptive (OC) users and postmenopausal well-trained women. Methods: This project is an observational controlled randomized counterbalanced study. One hundered and four (104) active and healthy women were selected to participate in the IronFEMME Study, 57 of which were eumenorrheic, 31 OC users and 16 postmenopausal. The project consisted of two sections carried out at the same time: iron metabolism (study I) and muscle damage (study II). For the study I, the exercise protocol consisted of an interval running test (eight bouts of 3 min at 85% of the maximal aerobic speed), whereas the study II protocol was an eccentric-based resistance exercise protocol (10 sets of 10 repetitions of plate-loaded barbell parallel back squats at 60% of their one repetition maximum (1RM) with 2 min of recovery between sets). In both studies, eumenorrheic participants were evaluated at three specific moments of the menstrual cycle: early-follicular phase, late-follicular phase and mid-luteal phase; OC users performed the trial at two moments: withdrawal phase and active pill phase. Lastly, postmenopausal women were only tested once, since their hormonal status does not fluctuate. The three-step method was used to verify the menstrual cycle phase: calendar counting, blood test confirmation, and urine-based ovulation kits. Blood samples were obtained to measure sex hormones, iron metabolism parameters, and muscle damage related markers. Discussion: IronFEMME Study has been designed to increase the knowledge regarding the influence of sex hormones on some aspects of the exercise-related female physiology. Iron metabolism and exercise-induced muscle damage will be studied considering the different reproductive status present throughout well-trained females’ lifespan.This publication has 92 references indexed in Scilit:
- Improved Mass Spectrometry Assay For Plasma Hepcidin: Detection and Characterization of a Novel Hepcidin IsoformPLOS ONE, 2013
- Effects of Estrogen Fluctuation during the Menstrual Cycle on the Response to Stretch-Shortening Exercise in FemalesBioMed Research International, 2013
- Estrogen regulates iron homeostasis through governing hepatic hepcidin expression via an estrogen response elementGene, 2012
- Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in HepatocytesPLOS ONE, 2012
- Executive summary of the Stages of Reproductive Aging Workshop + 10Menopause, 2012
- Four variants in transferrin and HFE genes as potential markers of iron deficiency anaemia risk: an association study in menstruating womenNutrition & Metabolism, 2011
- Common variants in TMPRSS6 are associated with iron status and erythrocyte volumeNature Genetics, 2009
- Hormone Therapy and Skeletal Muscle Strength: A Meta-AnalysisThe Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 2009
- Variants in TF and HFE Explain ∼40% of Genetic Variation in Serum-Transferrin LevelsAmerican Journal of Human Genetics, 2009
- The Menstrual CycleAnnals of the New York Academy of Sciences, 2008