Evaluation of Immobilization Techniques for the Fabrication of Nanomaterial-Based Amperometric Glucose Biosensors
- 31 December 2014
- journal article
- research article
- Published by Taylor & Francis Ltd in Analytical Letters
- Vol. 48 (8), 1297-1310
- https://doi.org/10.1080/00032719.2014.979364
Abstract
Eleven glucose biosensors were prepared by cross-linking, entrapment, and layer-by-layer assembly to investigate the influence of these immobilization methods on performance. The effects of separate nanozeolites combined with magnetic nanoparticles and multiwalled carbon nanotubes in the enzyme composition on the performance of glucose biosensors were compared. Cyclic voltammetric studies were carried out on the biosensors. Acrylonitrile copolymer/nanozeolite/carbon nanotube and acrylonitrile copolymer/nanozeolite/magnetic nanoparticle electrodes prepared by a cross-linking method showed the highest electroactivity. These results indicated that a synergistic effect occurred when multiwalled carbon nanotubes, magnetic nanoparticles, and nanozeolites were combined that greatly improved the electron transfer ability of the sensors. Amperometric measurements by the glucose oxidase electrodes were obtained that showed that the acrylonitrile copolymer/nanozeolite/carbon nanotube electrode was the most sensitive (10.959 microamperes per millimolar). The lowest detection limit for this biosensor was 0.02 millimolar glucose, with a linear dynamic range up to 3 millimolar. The response after thirty days was 81 percent of the initial current.Keywords
Funding Information
- The authors gratefully acknowledge the Bulgarian Ministry of Education and the National Science Fund (project DNTS–01/09) for their financial support and encouragement of the scientific research work in state universities.
This publication has 29 references indexed in Scilit:
- Immunofluorescent Analysis with Magnetic Nanoparticles for Simultaneous Determination of Antibiotic Residues in MilkAnalytical Letters, 2013
- Layer by layer assembly of glucose oxidase and thiourea onto glassy carbon electrode: Fabrication of glucose biosensorElectrochimica Acta, 2011
- Glucose biosensor based on covalent immobilization of enzyme in sol–gel composite film combined with Prussian blue/carbon nanotubes hybridBiosensors and Bioelectronics, 2011
- Microwave-assisted hydrothermal synthesis of nanozeolites with controllable sizeMicroporous and Mesoporous Materials, 2009
- Direct electrochemistry of glucose oxidase immobilized on a hexagonal mesoporous silica-MCM-41 matrixBioelectrochemistry, 2007
- Carbon-nanotubes doped polypyrrole glucose biosensorAnalytica Chimica Acta, 2005
- Design of an amperometric biosensor using polypyrrole-microgel composites containing glucose oxidaseBiosensors and Bioelectronics, 2004
- Electroactive Cytochrome c Multilayers within a Polyelectrolyte AssemblyAngewandte Chemie, 2004
- Fabrication of Controllable Ultrathin Hollow Shells by Layer-by-Layer Assembly of Exfoliated Titania Nanosheets on Polymer TemplatesChemistry of Materials, 2002
- Immobilization of flavoproteins on silicon: effect of cross-linker chain length on enzyme activityBiosensors and Bioelectronics, 1992