Can keratin scaffolds be used for creating three-dimensional cell cultures?
Open Access
- 1 January 2020
- journal article
- research article
- Published by Walter de Gruyter GmbH in Open Medicine
- Vol. 15 (1), 249-253
- https://doi.org/10.1515/med-2020-0031
Abstract
Three-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.Keywords
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