Characterizing the microbiome of ectoparasitic louse flies feeding on migratory raptors
Open Access
- 3 June 2020
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 15 (6), e0234050
- https://doi.org/10.1371/journal.pone.0234050
Abstract
Louse flies (Diptera: Hippoboscidae) are obligate ectoparasites that often cause behavioral, pathogenic, and evolutionary effects on their hosts. Interactions between ectoparasites and avian hosts, especially migrating taxa, may influence avian pathogen spread in tropical and temperate ecosystems and affect long-term survival, fitness and reproductive success. The purpose of this study was to characterize the vector-associated microbiome of ectoparasitic louse flies feeding on migrating raptors over the fall migration period. Surveys for louse flies occurred during fall migration (2015-2016) at a banding station in Pennsylvania, United States; flies were collected from seven species of migrating raptors, and we sequenced their microbial (bacteria and archaea) composition using high-throughput targeted amplicon sequencing of the 16S rRNA gene (V4 region). All louse flies collected belonged to the same species, Icosta americana. Our analysis revealed no difference in bacterial communities of louse flies retrieved from different avian host species. The louse fly microbiome was dominated by a primary endosymbiont, suggesting that louse flies maintain a core microbial structure despite receiving blood meals from different host species. Thus, our findings highlight the importance of characterizing both beneficial and potentially pathogenic endosymbionts when interpreting how vector-associated microbiomes may impact insect vectors and their avian hosts.Keywords
This publication has 77 references indexed in Scilit:
- Characterization of the Bacterial Community Associated with Larvae and Adults ofAnoplophora chinensisCollected in Italy by Culture and Culture-Independent MethodsBioMed Research International, 2013
- Winter Temperature Affects the Prevalence of Ticks in an Arctic SeabirdPLOS ONE, 2013
- phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census DataPLOS ONE, 2013
- MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and UsabilityMolecular Biology and Evolution, 2013
- Pathogenicity of and plant immunity to soft rot pectobacteriaFrontiers in Plant Science, 2013
- Microbiome influences on insect host vector competenceTrends in Parasitology, 2011
- Parasitism and the evolutionary ecology of animal personalityPhilosophical Transactions Of The Royal Society B-Biological Sciences, 2010
- Global patterns of 16S rRNA diversity at a depth of millions of sequences per sampleProceedings of the National Academy of Sciences of the United States of America, 2010
- Isolation, Pure Culture, and Characterization of “ Candidatus Arsenophonus arthropodicus,” an Intracellular Secondary Endosymbiont from the Hippoboscid Louse Fly Pseudolynchia canariensisApplied and Environmental Microbiology, 2006
- A mathematical model for the global spread of influenzaMathematical Biosciences, 1985