MicroRNA-148b-3p and MicroRNA-25-3p Are Overexpressed in Fetuses with Late-Onset Fetal Growth Restriction
- 1 September 2020
- journal article
- research article
- Published by S. Karger AG in Fetal Diagnosis and Therapy
- Vol. 47 (9), 665-674
- https://doi.org/10.1159/000507619
Abstract
Objective:It was the aim of this study to describe a micro-RNA (miRNA) profile characteristic of late-onset fetal growth restriction (FGR) and to investigate the pathways involved in their biochemical action.Methods:In this prospective study, 25 fetuses (16 normal and 9 with FGR [estimated fetal weight <10th centile plus cerebroplacental ratio <0.6765 multiples of the median]) were evaluated with Doppler ultrasound after 36 weeks. Afterwards, for every fetus, plasma from umbilical vein blood was collected at birth, miRNA was extracted, and full miRNA sequencing was performed. Subsequently, comparisons were done in order to obtain those miRNAs that were differentially expressed.Results:The FGR fetuses expressed upregulation of two miRNAs: miR-25-3p and, especially, miR-148b-3p, a miRNA directly involved in Schwann cell migration, neuronal plasticity, and energy metabolism (p= 0.0072,p= 0.0013).Conclusions:FGR fetuses express a different miRNA profile, which includes overexpression of miR-25-3p and miR-148b-3p. This information might improve our understanding of the pathophysiological processes involved in late-onset FGR. Future validation and feasibility studies will be required to propose miRNAs as a valid tool in the diagnosis and management of FGR.This publication has 57 references indexed in Scilit:
- The Subread aligner: fast, accurate and scalable read mapping by seed-and-voteNucleic Acids Research, 2013
- The Impact of Hemolysis on Cell-Free microRNA BiomarkersFrontiers in Genetics, 2013
- miRNA-132: a dynamic regulator of cognitive capacityBrain Structure and Function, 2012
- microRNA-132 regulates dendritic growth and arborization of newborn neurons in the adult hippocampusProceedings of the National Academy of Sciences of the United States of America, 2010
- The levels of hypoxia-regulated microRNAs in plasma of pregnant women with fetal growth restrictionPlacenta, 2010
- Origins and Mechanisms of miRNAs and siRNAsCell, 2009
- MicroRNAs: Target Recognition and Regulatory FunctionsCell, 2009
- Most mammalian mRNAs are conserved targets of microRNAsGenome Research, 2008
- An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAPProceedings of the National Academy of Sciences of the United States of America, 2008
- Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA TargetsCell, 2005