Plant Cell Identity in the Era of Single-Cell Transcriptomics
- 23 November 2021
- journal article
- review article
- Published by Annual Reviews in Annual Review of Genetics
- Vol. 55 (1), 479-496
- https://doi.org/10.1146/annurev-genet-071719-020453
Abstract
High-throughput single-cell transcriptomic approaches have revolutionized our view of gene expression at the level of individual cells, providing new insights into their heterogeneity, identities, and functions. Recently, technical challenges to the application of single-cell transcriptomics to plants have been overcome, and many plant organs and tissues have now been subjected to analyses at single-cell resolution. In this review, we describe these studies and their impact on our understanding of the diversity, differentiation, and activities of plant cells. We particularly highlight their impact on plant cell identity, including unprecedented views of cell transitions and definitions of rare and novel cell types. We also point out current challenges and future opportunities for the application and analyses of single-cell transcriptomics in plants. Expected final online publication date for the Annual Review of Genetics, Volume 55 is November 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.Keywords
This publication has 109 references indexed in Scilit:
- Unleashing the potential of the root hair cell as a single plant cell type model in root systems biologyFrontiers in Plant Science, 2013
- A Gene Regulatory Network for Root Epidermis Cell Differentiation in ArabidopsisPLoS Genetics, 2012
- Central dogma at the single-molecule level in living cellsNature, 2011
- A Simple Method for Gene Expression and Chromatin Profiling of Individual Cell Types within a TissueDevelopmental Cell, 2010
- Shifting the genomic gold standard for the prokaryotic species definitionProceedings of the National Academy of Sciences of the United States of America, 2009
- Transcriptome Analysis of Arabidopsis Wild-Type and gl3–sst sim Trichomes Identifies Four Additional Genes Required for Trichome DevelopmentMolecular Plant, 2009
- A transcriptome atlas of rice cell types uncovers cellular, functional and developmental hierarchiesNature Genetics, 2009
- Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cellsBMC Genomics, 2007
- Stochastic mRNA Synthesis in Mammalian CellsPLoS Biology, 2006
- Human cell type diversity, evolution, development, and classification with special reference to cells derived from the neural crestBiological Reviews, 2006