SPTBN1 suppresses the progression of epithelial ovarian cancer via SOCS3-mediated blockade of the JAK/STAT3 signaling pathway
Open Access
- 8 June 2020
- journal article
- research article
- Published by Impact Journals, LLC in Aging
- Vol. 12 (11), 10896-10911
- https://doi.org/10.18632/aging.103303
Abstract
SPTBN1 plays an anticancer role in many kinds of tumors and participates in the chemotherapeutic resistance of epithelial ovarian cancer (EOC). Here, we reported that lower SPTBN1 expression was significantly related to advanced EOC stage and shorter progression-free survival. SPTBN1 expression was also higher in less invasive EOC cell lines. Moreover, SPTBN1 decreased the migration ability of the EOC cells A2780 and HO8910 and inhibited the growth of EOC cells in vitro and tumor xenografts in vivo. SPTBN1 suppression increased the epithelial mesenchymal transformation marker Vimentin while decreasing E-cadherin expression. By analyzing TCGA data and immunohistochemistry staining of tumor tissue, we found that SPTBN1 and SOCS3 were positively coexpressed in EOC patients. SOCS3 overexpression or JAK2 inhibition decreased the proliferation and migration of EOC cells as well as the expression of p-JAK2, p-STAT3 and Vimentin, which were enhanced by the downregulation of SPTBN1, while E-cadherin expression was also reversed. It was also verified in mouse embryonic fibroblasts (MEFs) that loss of SPTBN1 activated the JAK/STAT3 signaling pathway with suppression of SOCS3. Our results suggest that SPTBN1 suppresses the progression of epithelial ovarian cancer via SOCS3-mediated blockade of the JAK/STAT3 signaling pathway.Keywords
This publication has 42 references indexed in Scilit:
- SOCS, inflammation, and cancerJAK-STAT, 2013
- Enhancement of leptin receptor signaling by SOCS3 deficiency induces development of gastric tumors in miceOncogene, 2012
- Bisphenol A induces leptin receptor expression, creating more binding sites for leptin, and activates the JAK/Stat, MAPK/ERK and PI3K/Akt signalling pathways in human ovarian cancer cellToxicology Letters, 2012
- Spectrin αII and βII tetramers contribute to platinum anticancer drug resistance in ovarian serous adenocarcinomaInternational Journal of Cancer, 2011
- SOCS3 regulates p21 expression and cell cycle arrest in response to DNA damageCellular Signalling, 2008
- Hepatocellular cancer arises from loss of transforming growth factor beta signaling adaptor protein embryonic liver fodrin through abnormal angiogenesisHepatology, 2008
- Inactivation of TGF-β signaling in lung cancer results in increased CDK4 activity that can be rescued by ELFBiochemical and Biophysical Research Communications, 2006
- Deletion of the SOCS3 Gene in Liver Parenchymal Cells Promotes Hepatitis–Induced HepatocarcinogenesisGastroenterology, 2006
- Critical interactions between TGF-β signaling/ELF, and E-cadherin/β-catenin mediated tumor suppressionOncogene, 2006
- Suppression of IL-6 production and proliferation by blocking STAT3 activation in malignant soft tissue tumor cellsCancer Letters, 2006