Novel extra cellular-like matrices to improve human ovarian grafting
- 24 July 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Journal of Assisted Reproduction and Genetics
- Vol. 37 (9), 2105-2117
- https://doi.org/10.1007/s10815-020-01832-4
Abstract
Purpose To investigate if human ovarian grafting with pure virgin human recombinant collagen type-1 from bioengineered plant lines (CollPlant™) or small intestine submucosa (SIS) yields better implantation results for human ovarian tissue and which method benefits more when combined with the host melatonin treatment and graft incubation with biological glue + vitamin E + vascular endothelial growth factor-A. Methods Human ovarian tissue wrapped in CollPlant or SIS was transplanted into immunodeficient mice with/without host/graft treatment. The tissue was assessed by follicle counts (including atretic), for apoptosis evaluation by terminal deoxynucleotidyl transferase assay and for immunohistochemical evaluation of neovascularization by platelet endothelial cell adhesion molecule (PECAM) expression, and for identification of proliferating granulosa cells by Ki67 expression. Results Human ovarian tissue transplanted with CollPlant or SIS fused with the surrounding tissue and promoted neovascularization. In general, implantation with CollPlant even without additives promoted better results than with SIS: significantly higher number of recovered follicles, significantly fewer atretic follicles, and significantly more granulosa cell proliferation. Moreover, results with CollPlant alone seemed to be at least as good as those after host and graft treatments. Conclusions CollPlant is a biomaterial without any potential risks, and grafting ovarian tissue with CollPlant is easy and the procedure may be easily modified, with limited or no foreseeable risks, for auto-transplantation in cancer survivors. Further studies are needed using other novel methods capable of enhancing neovascularization and reducing apoptosis and follicle atresia.Keywords
Funding Information
- Israel Ministry of Health (3-6098-00000)
This publication has 40 references indexed in Scilit:
- Live birth in a woman without ovaries after autograft of frozen-thawed ovarian tissue combined with growth factorsJournal of Ovarian Research, 2013
- Enhancement of Neoangiogenesis and Follicle Survival by Sphingosine-1-Phosphate in Human Ovarian Tissue XenotransplantsPLOS ONE, 2011
- Angiogenesis of the Frozen-Thawed Human Fetal Ovarian Tissue at the Early Stage after Xenotransplantation and the Positive Effect of Salviae miltiorrhizaeThe Anatomical Record, 2010
- Both host and graft vessels contribute to revascularization of xenografted human ovarian tissue in a murine modelFertility and Sterility, 2010
- Production of Bioactive, Post-Translationally Modified, Heterotrimeric, Human Recombinant Type-I Collagen in Transgenic TobaccoBiomacromolecules, 2009
- Expression of VEGF and neural repair after alprostadil treatment in a rat model of sciatic nerve crush injuryNeurology India, 2009
- Newly identified biologically active and proteolysis-resistant VEGF-A isoform VEGF111 is induced by genotoxic agentsThe Journal of cell biology, 2007
- Galα(1,3)Gal Epitope in Porcine Small Intestinal SubmucosaTissue Engineering, 2000
- Histology after dural grafting with small intestinal submucosaSurgical Neurology, 1996
- Apoptosis and ovarian functionReviews of Reproduction, 1996