Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration
Open Access
- 10 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-12
- https://doi.org/10.1038/s41598-020-80624-3
Abstract
In mammals, organ induction occurs only during embryonic development except for hair follicles (HFs). However, HF-resident epithelial stem cells (HFSCs), which are responsible for repetitive HF regeneration, are not fully characterized. Here, we establish in vitro culture systems that are capable of controlling the ability of HFSCs to regenerate HFs. Based on a method that precisely controlled the number of HFs for regeneration, functional analysis revealed that CD34/CD49f/integrin β5 (Itgβ5)-triple-positive (CD34+/CD49f+/Itgβ5+) cells have multipotency and functional significance for continual hair regeneration. In native HFs, these cells reside in the uppermost area of the bulge region, which is surrounded by tenascin in mice and humans. This study unveils the subpopulation of HFSCs responsible for long-term hair cycling of HFs regenerated from bioengineered HF germ, suggesting the presence of functional heterogeneity among bulge HFSCs and the utility of our culture system to achieve HF regenerative therapy.Funding Information
- Japan Society for the Promotion of Science (JP16H07454)
This publication has 38 references indexed in Scilit:
- Fully functional hair follicle regeneration through the rearrangement of stem cells and their nichesNature Communications, 2012
- Genome-wide Maps of Histone Modifications Unwind In Vivo Chromatin States of the Hair Follicle LineageCell Stem Cell, 2011
- TCF/Lef1 activity controls establishment of diverse stem and progenitor cell compartments in mouse epidermisThe EMBO Journal, 2011
- The Basement Membrane of Hair Follicle Stem Cells Is a Muscle Cell NicheCell, 2011
- Kruppel-like Factor 4 (Klf4) Prevents Embryonic Stem (ES) Cell Differentiation by Regulating Nanog Gene ExpressionOnline Journal of Public Health Informatics, 2010
- Distinct Self-Renewal and Differentiation Phases in the Niche of Infrequently Dividing Hair Follicle Stem CellsCell Stem Cell, 2009
- Fully functional bioengineered tooth replacement as an organ replacement therapyProceedings of the National Academy of Sciences of the United States of America, 2009
- Lrig1 Expression Defines a Distinct Multipotent Stem Cell Population in Mammalian EpidermisCell Stem Cell, 2009
- A Two-Step Mechanism for Stem Cell Activation during Hair RegenerationCell Stem Cell, 2009
- Blimp1 Defines a Progenitor Population that Governs Cellular Input to the Sebaceous GlandCell, 2006