High-speed compressed-sensing fluorescence lifetime imaging microscopy of live cells
- 11 January 2021
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 118 (3)
- https://doi.org/10.1073/pnas.2004176118
Abstract
We present high-resolution, high-speed fluorescence lifetime imaging microscopy (FLIM) of live cells based on a compressed sensing scheme. By leveraging the compressibility of biological scenes in a specific domain, we simultaneously record the time-lapse fluorescence decay upon pulsed laser excitation within a large field of view. The resultant system, referred to as compressed FLIM, can acquire a widefield fluorescence lifetime image within a single camera exposure, eliminating the motion artifact and minimizing the photobleaching and phototoxicity. The imaging speed, limited only by the readout speed of the camera, is up to 100 Hz. We demonstrated the utility of compressed FLIM in imaging various transient dynamics at the microscopic scale.Keywords
Funding Information
- HHS | NIH | National Eye Institute (R01EY029397)
- HHS | NIH | National Institute of General Medical Sciences (R35GM128761)
- HHS | NIH | National Institute of Biomedical Imaging and Bioengineering (R21EB028375)
- NSF | ENG | Division of Chemical, Bioengineering, Environmental, and Transport Systems (1652150)
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