Genome-scale deconvolution of RNA structure ensembles
- 22 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 18 (3), 249-252
- https://doi.org/10.1038/s41592-021-01075-w
Abstract
RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3′ untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.Keywords
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