Objective — To conduct studies on the application effect of humanistic care in the operation room. Methods — A total of 90 patients who received surgical treatment in our hospital from January 2021 to May 2021 were selected and divided into experimental group and control group based on the order of admission, with 45 patients in each group. The patients in the control group were treated with routine surgical nursing, and the patients in the experimental group were treated with humanistic care on the basis of routine surgical nursing. Comparison was made in the application effect of the two groups. Results — The scores of Self-Rating Depression Scale and Self-Rating Anxiety Scale in two groups demonstrated significant decreasing. The scores of the Self-Rating Depression Scale and Self-Rating Anxiety Scale were significantly reduced in the two groups after psychological nursing intervention. Conclusions — Effective humanistic care can not only contributes to the relief of anxiety or depression and other unhealthy emotions of patients, but also serves as an important way to maintain a good doctor-patient relationship. Humanistic care has certain application value in clinical applications.