Mesenchymal Stem Cell-Derived Extracellular Vesicles for Corneal Wound Repair
Open Access
- 9 December 2019
- journal article
- research article
- Published by Hindawi Limited in Stem Cells International
- Vol. 2019, 1-9
- https://doi.org/10.1155/2019/5738510
Abstract
With the immunoregulation potential, mesenchymal stem cells (MSCs) have been used for tissue regeneration by relieving inflammation in the injured tissues. When this repair process is interfered by immune disorders or pathological angiogenesis, the delays in corneal epithelial wound healing can lead to a persistent epithelial defect. Stem cell-derived extracellular vesicles (EVs), which carry abundant bioactive molecules from stem cells, have provided an alternative to regeneration therapy. In this study, we aimed to investigate if EVs from human placenta-derived MSCs (hP-MSCs) could ameliorate alkali injury of the cornea in the mouse model. 33.33 μg/μL EVs in 10 μL PBS were applied to the cornea. Repeat application three times, and 100 μg EVs (in 30 μL PBS) in total were administrated per day for two weeks. Our results revealed that EVs from hP-MSCs had preferable functions including enhancing proliferation and anti-inflammation and suppressing apoptosis of corneal epithelial cells. Furthermore, hP-MSC-derived EVs ameliorated mouse corneal wound healing by inhibiting angiogenesis and inflammation. Taken together, our current data suggested that hP-MSC-derived EVs have the beneficial effects of corneal wound healing, which provide alternative cell-free therapy with great practical value.Funding Information
- Key Projects of Tianjin Science and Technology Support Program (18YFZCSY00010, 81671734, 2017YFA0103200)
This publication has 36 references indexed in Scilit:
- Ocular Drug Delivery Nanowafer with Enhanced Therapeutic EfficacyACS Nano, 2015
- Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance ImagingAnalytical Chemistry, 2014
- Identification of retinal ganglion cell neuroprotection conferred by platelet-derived growth factor through analysis of the mesenchymal stem cell secretomeBrain, 2013
- Exosomes Mediate the Cytoprotective Action of Mesenchymal Stromal Cells on Hypoxia-Induced Pulmonary HypertensionCirculation, 2012
- Targeted deletion of Vegfa in adult mice induces vision lossJCI Insight, 2012
- Mesenchymal Stem Cell-Derived Microvesicles Protect Against Acute Tubular InjuryJournal of the American Society of Nephrology, 2009
- VEGF Inhibition and Renal Thrombotic MicroangiopathyThe New England Journal of Medicine, 2008
- Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivoProceedings of the National Academy of Sciences of the United States of America, 2002
- Corneal neovascularizationCurrent Opinion in Opthalmology, 2001
- Intact corneal epithelium is essential for the prevention of stromal haze after laser assisted in situ keratomileusisBritish Journal of Ophthalmology, 2001