Monoclonal antibody covalently coupled to liposomes: Specific targeting to cells
- 1 January 1981
- journal article
- research article
- Published by Wiley in Journal of Supramolecular Structure and Cellular Biochemistry
- Vol. 16 (3), 243-258
- https://doi.org/10.1002/jsscb.1981.380160305
Abstract
We have evaluated optimal conditions for coupling monoclonal antibody to small unilamellar lipisomes. Coupling of an IgG2a monoclonal anti-β2-microglobulin antibody, which reacts with human cells, was examined in detail. Liposomes were composed of dipalmitoyl lecithin and cholesterol, and variable quantities of phosphatidylethanolamine substituted with the heterobifunctional cross-linking reagent N-hydroxysuccinimidyl 3-(2-pyridyldithio) propionate (SPDP). They were reacted with antibody derivatized with the same reagent at a 5- to 20-fold molar excess, and activated by mild reduction. This degree of SPDP modification had no effect on the capacity of the antibody to bind to its target antigen. More than 40% of antibody could be reproducibly bound to liposomes, resulting in the coupling of from 1 to 10 antibody molecules per liposome (mean diameter.580 Å). The coupling reaction did not lead to loss of carboxyfluorescein encapsulated within liposomes. At least 80% of liposomes carried nondenatured antibody, as confirmed by precipitation of liposomes and encapsulated carboxyfluorescein by Staphylococcus aureus, strain Cowan I. The liposome-coupled antibody retained its immunological specificity: only cells expressing human β2-microglobulin bound liposomes in vitro, and the binding was inhibited by the free antibody in solution. Results with antibodies of different antigenic specificity confirm that the technique can be generally applied.Keywords
This publication has 21 references indexed in Scilit:
- Targeting to cells of fluorescent liposomes covalently coupled with monoclonal antibody or protein ANature, 1980
- Antibody Targeting of Liposomes: Cell Specificity Obtained by Conjugation of F(ab′) 2 to Vesicle SurfaceScience, 1980
- A comparison of triton X‐100 and the bile salt taurocholate as micellar ionophores or fusogens in phospholipid vesicular membranesFEBS Letters, 1980
- Attachment to membranes of exogenous immunoglobulin conjugated to a hydrophobic anchorBiochemical and Biophysical Research Communications, 1979
- Formation of unilamellar lipid vesicles of controllable dimensions by detergent dialysisBiochemistry, 1979
- Preservation of antimyosin antibody activity after covalent coupling to liposomesBiochemical and Biophysical Research Communications, 1979
- Binding of immunoglobulin G to phospholipid vesicles by sonicationBiochemistry, 1979
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Effect of immunoglobulin class and affinity on the initiation of complement-dependent damage to liposomal model membranes sensitized with dinitrophenylated phospholipidsBiochemistry, 1973
- Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific ActivityNature, 1962