Effect of naive and cancer-educated fibroblasts on colon cancer cell circadian growth rhythm
Open Access
- 27 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cell Death & Disease
- Vol. 11 (4), 1-15
- https://doi.org/10.1038/s41419-020-2468-2
Abstract
Opportunistic modification of the tumour microenvironment by cancer cells enhances tumour expansion and consequently eliminates tumour suppressor components. We studied the effect of fibroblasts on the circadian rhythm of growth and protein expression in colon cancer HCT116 cells and found diminished oscillation in the proliferation of HCT116 cells co-cultured with naive fibroblasts, compared with those co-cultured with tumour-associated fibroblasts (TAFs) or those cultured alone, suggesting that TAFs may have lost or gained factors that regulate circadian phenotypes. Based on the fibroblast paracrine factor analysis, we tested IL6, which diminished HCT116 cell growth oscillation, inhibited early phase cell proliferation, increased early phase expression of the differentiation markers CEA and CDX2, and decreased early phase ERK5 phosphorylation. In conclusion, our data demonstrate how the cancer education of naive fibroblasts influences the circadian parameters of neighbouring cancer cells and highlights a putative role for IL6 as a novel candidate for preoperative treatments.Keywords
This publication has 50 references indexed in Scilit:
- Clock Genes and CancerIntegrative Cancer Therapies, 2009
- A Role for the Clock Gene Per1 in Prostate CancerCancer Research, 2009
- Progress in the epidemiological understanding of gene–environment interactions in major diseases: cancerComptes Rendus Biologies, 2007
- Stimulation of angiogenesis by Ras proteinsBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 2004
- The origin of the myofibroblasts in breast cancer. Recapitulation of tumor environment in culture unravels diversity and implicates converted fibroblasts and recruited smooth muscle cells.JCI Insight, 1995
- Autocrine and paracrine growth regulation of human breast cancerJournal of Steroid Biochemistry, 1986
- Circadian Rhythm in Uptake of Tritiated Thymidine by Kidney, Parotid, and Duodenum of Isoproterenol-Treated MiceScience, 1972
- Circadian Rhythms in Rats: Effects of Random LightingScience, 1966
- CHANGES IN THE TISSUE SURROUNDING A GROWING TUMOR AND THE SIGNIFICANCE OF THE "PRECANCEROUS STATE."The Journal of Experimental Medicine, 1912
- LYMPHOMA, A BENIGN TUMOR REPRESENTING A LYMPH GLAND IN STRUCTUREThe Journal of Experimental Medicine, 1899