Bio nanocomposites of graphene oxide with carboxymethyl guargum: fabrication and characterization and application for type 1 diabetes
- 4 October 2021
- journal article
- research article
- Published by IOP Publishing in Biomedical Materials
- Vol. 16 (6), 065025
- https://doi.org/10.1088/1748-605x/ac2c8e
Abstract
Islet cells transplantation has limitations like low survivability, which can be overcome by using extracellular matrix mimicking three dimensional (3D) scaffolds, which supports the growth and proliferation of seeded cells. This study was aimed to investigate the role of novel 3D Carboxymethyl guargum (CMGG) nanocomposite with reduced graphene oxide (rGO) for proliferation of pancreatic islet cells (RIN-5F) and rate of insulin secretion of RIN-5F cells. SEM and FTIR results have demonstrated good porosity and the chemical interactions between CMGG and rGO. Mechanical testing and TGA of nanofibers have shown good tensile strength and thermal stability with rGO in the nanocomposite. These scaffolds demonstrated in vitro biocompatibility with acceptable ranges of biodegradability and hemocompatibility. The in vitro cell proliferation and viability of RIN-5F cells on 3D CMGG nanofibers have significantly increased compared to 2D cell control. Moreover, the glucose dependent insulin secretion of RIN-5F cells on CMGG nanocomposite has significantly increased upto 4-5 folds than cells on 2D cell control. The biomaterials used in this 3D nanofiber scaffold have shown to be biodegradable and hemocompatible and can be a promising platform for the proliferation and secretion of insulin from beta cells and can be effectively used in transplantation type-1 diabetes.Keywords
This publication has 28 references indexed in Scilit:
- Tuning Fe 3 O 4 nanoparticle dispersion through pH in PVA/guar gum/electrospun membranesCarbohydrate Polymers, 2015
- Enhanced Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Graphene Oxide-Incorporated Electrospun Poly(lactic-co-glycolic acid) Nanofibrous MatsACS Applied Materials & Interfaces, 2015
- Safety concerns to application of graphene compounds in pharmacy and medicineDARU Journal of Pharmaceutical Sciences, 2014
- Immunosuppression for pancreas transplantation with an emphasis on antibody induction strategies: review and perspectiveExpert Review of Clinical Immunology, 2013
- Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineeringBiomaterials, 2013
- Hyaluronic acid based scaffolds for tissue engineering—A reviewCarbohydrate Polymers, 2013
- Evolution ofβ-Cell Replacement Therapy in Diabetes Mellitus: Pancreas TransplantationDiabetes Technology & Therapeutics, 2011
- Three-dimensional Cultures of Rat Pancreatic RIN-5F Cells in Porous PLGA-collagen Hybrid ScaffoldsJournal of Bioactive and Compatible Polymers, 2009
- Five-Year Follow-Up After Clinical Islet TransplantationDiabetes, 2005
- Graft copolymerization of 2‐hydroxyethylmethacrylate onto chitosan films and their blood compatibilityJournal of Applied Polymer Science, 1994