Long noncoding RNA and protein abundance in lncRNPs
Open Access
- 15 September 2021
- journal article
- review article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 27 (12), 1427-1440
- https://doi.org/10.1261/rna.078971.121
Abstract
Although long noncoding RNAs (lncRNAs) are generally expressed at low levels, emerging evidence has revealed that many play important roles in gene regulation by a variety of mechanisms as they engage with proteins. Given that the abundance of proteins often greatly exceeds that of their interacting lncRNAs, quantification of the relative abundance, or even the exact stoichiometry in some cases, within lncRNA-protein complexes is helpful for understanding of the mechanism(s) of action of lncRNAs. We discuss methods used to examine lncRNA and protein expression at the single cell, sub-cellular and sub-organelle levels, the average and local lncRNA concentration in cells, as well as how lncRNAs can modulate the functions of their interacting proteins even at a low stoichiometric concentration.Keywords
Funding Information
- National Natural Science Foundation of China (31725009, 31830108)
- Chinese Academy of Sciences (YSBR-009)
- Shanghai Municipal Commission for Science and Technology (20JC1410300)
- oward Hughes Medical Institute (55008728)
- XPLORER PRIZE
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