Mass collection of magnetotactic bacteria from the permanently stratified ferruginous Lake Pavin, France
Open Access
- 9 March 2021
- journal article
- research article
- Published by Wiley in Environmental Microbiology
- Vol. 24 (2), 721-736
- https://doi.org/10.1111/1462-2920.15458
Abstract
Obtaining high biomass yields of specific microorganisms for culture‐independent approaches is a challenge faced by scientists studying organism's recalcitrant to laboratory conditions and culture. This difficulty is highly decreased when studying magnetotactic bacteria (MTB) since their unique behaviour allows their enrichment and purification from other microorganisms present in aquatic environments. Here, we use Lake Pavin, a permanently stratified lake in the French Massif Central, as a natural laboratory to optimize collection and concentration of MTB that thrive in the water column and sediments. A method is presented to separate MTB from highly abundant abiotic magnetic particles in the sediment of this crater lake. For the water column, different sampling approaches are compared such as in situ collection using a Niskin bottle and online pumping. By monitoring several physicochemical parameters of the water column, we identify the ecological niche where MTB live. Then, by focusing our sampling at the peak of MTB abundance, we show that the online pumping system is the most efficient for fast recovering of large volumes of water at a high spatial resolution, which is necessary considering the sharp physicochemical gradients observed in the water column. Taking advantage of aerotactic and magnetic MTB properties, we present an efficient method for MTB concentration from large volumes of water. Our methodology represents a first step for further multidisciplinary investigations of the diversity, metagenomic and ecology of MTB populations in Lake Pavin and elsewhere, as well as chemical and isotopic analyses of their magnetosomes.Funding Information
- Agence Nationale de la Recherche (ANR‐18‐CE31‐0003, ANR‐19‐CE01‐0005)
- Centre National de la Recherche Scientifique
This publication has 74 references indexed in Scilit:
- Integrating niche-based process and spatial process in biogeography of magnetotactic bacteriaScientific Reports, 2013
- Insight into the Evolution of Magnetotaxis in Magnetospirillum spp., Based on mam Gene PhylogenyApplied and Environmental Microbiology, 2012
- Newly Isolated but Uncultivated Magnetotactic Bacterium of the Phylum Nitrospirae from Beijing, ChinaApplied and Environmental Microbiology, 2012
- Biogeochemical modelling of anaerobic vs. aerobic methane oxidation in a meromictic crater lake (Lake Pavin, France)Applied Geochemistry, 2011
- Magnetotactic Bacteria Containing Phosphorus-Rich Inclusion BodiesMicroscopy and Microanalysis, 2011
- Genomics, Genetics, and Cell Biology of Magnetosome FormationAnnual Review of Microbiology, 2009
- Toward Cloning of the Magnetotactic Metagenome: Identification of Magnetosome Island Gene Clusters in Uncultivated Magnetotactic Bacteria from Different Aquatic SedimentsApplied and Environmental Microbiology, 2009
- Comparative analysis of magnetosome gene clusters in magnetotactic bacteria provides further evidence for horizontal gene transferEnvironmental Microbiology, 2009
- Hydrological budget, carbon sources and biogeochemical processes in Lac Pavin (France): Constraints from δ18O of water and δ13C of dissolved inorganic carbonApplied Geochemistry, 2008
- Magnetosome formation in prokaryotesNature Reviews Microbiology, 2004