Soybean is susceptible to saline stress and reduced yield loss. Organic fertilization has several beneficial effects on agricultural crop fields. To minimize the deleterious effects of the salinity organic fertilization is using which can provide economic yield under saline conditions. Therefore, a greenhouse research was conducted at the Department of Environmental Dynamics and Management, Hiroshima University, Japan to find out the role of compost in alleviating the adverse effects of salinity stress on soybean. The nitrogen-fixing activity was estimated by using gas chromatograph. The results revealed that salinity stress reduced the biological nitrogen fixation and specific nodule activity. Our results show that, there was a corresponding improve in nitrogen fixation with compost application.