Curcumin-crosslinked acellular bovine pericardium for the application of calcification inhibition heart valves
- 1 July 2020
- journal article
- research article
- Published by IOP Publishing in Biomedical Materials
- Vol. 15 (4), 045002
- https://doi.org/10.1088/1748-605X/ab6f46
Abstract
Glutaraldehyde (GA) crosslinked bovine or porcine pericardium tissues exhibit high cell toxicity and calcification in the construction of bioprosthetic valves, which accelerate the failure of valve leaflets and motivate the exploration for alternatives. Polyphenols, including curcumin, procyanidin and quercetin, etc, have showed great calcification inhibition potential in crosslinking collagen and elastin scaffolds. Herein, we developed an innovative phenolic fixing technique by using curcumin as the crosslinking reagent for valvular materials. X-ray photoelectron spectroscopy and Fourier transform infrared spectrometry assessments confirmed the hydrogen bond between curcumin and acellular bovine pericardium. Importantly, the calcification inhibition capability of the curcumin-crosslinked bovine pericardium was proved by the dramatically reduced Ca2+ content in the curcumin-fixed group in in vitro assay, a juvenile rat subcutaneous implants model, as well as an osteogenic differentiation model. In addition, the results showed that the curcumin-fixed bovine pericardium exhibited better performance in the areas of mechanical performance, hemocompatibility and cytocompatibility, in comparison with the GA group and the commercialized product. In summary, we demonstrated that curcumin was a feasible crosslinking reagent to fix acellular bovine pericardium, which showed great potential for biomedical applications, particularly in cardiovascular biomaterials with calcification inhibition capacity.Funding Information
- Natural Science Foundation of China ((no. 81530059, no. 31800815, no. 31771059))
- Science and Technology Support Program of Tianjin (19YFZCSY00520)
- Chinese Academy of Medical Sciences, CAMS Initiative for Innovative Medicine (2017-12M-3-002)
- Science & Technology Projects of Tianjin of China (18JCQNJC14200)
- Fundamental Research Funds for the Central Universities (3332018119)
- Special Program for High-Tech Leader & Team of Tianjin Government
- Tianjin Innovation Promotion Plan Key Innovation Team of Immunoreactive Biomaterials
This publication has 49 references indexed in Scilit:
- Fucoidan/VEGF-based surface modification of decellularized pulmonary heart valve improves the antithrombotic and re-endothelialization potential of bioprosthesesBiomaterials, 2018
- Biomaterial characterization of off-the-shelf decellularized porcine pericardial tissue for use in prosthetic valvular applicationsJournal of Tissue Engineering and Regenerative Medicine, 2018
- Natural Extracellular Matrix for Cellular and Tissue BiomanufacturingACS Biomaterials Science & Engineering, 2016
- A novel crosslinking method for improved tear resistance and biocompatibility of tissue based biomaterialsBiomaterials, 2015
- Polymeric heart valves for surgical implantation, catheter-based technologies and heart assist devicesBiomaterials, 2015
- Design and Testing of a Pulsatile Conditioning System for Dynamic Endothelialization of Polyphenol-Stabilized Tissue Engineered Heart ValvesCardiovascular Engineering and Technology, 2010
- Evolving Concepts of Cardiac Valve DynamicsCirculation, 2008
- The pathology of fresh and cryopreserved homograft heart valves: An analysis of forty explanted homograft valvesThe Journal of Thoracic and Cardiovascular Surgery, 2002
- Progression of Aortic Valve CalcificationCirculation, 2001
- Glutaraldehyde as a crosslinking agent for collagen-based biomaterialsJournal of Materials Science: Materials in Medicine, 1995