Study of Heat Shock Protein (Hsp) 70 gene expression of Emilia sonchifolia L. and Sphagneticola trilobata L. in Universitas Indonesia, Depok and Kebun Raya Cibodas

Abstract

The temperature of earth surface is generally different according to altitude. Universitas Indonesia (UI) is located at 50–140 masl with an average temperature of 28.6 °C, while Kebun Raya Cibodas (KRC) located at 1.300–1.425 masl with an average temperature of 20.06 °C. Temperature differences are thought to affect plant responses, such as the expression of heat shock protein (Hsp) 70 genes. The research aims to find out the expression of Hsp70 genes on Emilia sonchifolia and Sphagneticola trilobata collected from UI and KRC. The study was conducted by isolating RNA from young leaves, which then converted into cDNA. The cDNA product was further amplified by polymerase chain reaction (PCR) using Hsp70 Arabidopsis thaliana primer. The amplification products were then sequenced and analyzed by in-silico. The results of amplification show that there is a band with approximately 250 bp in all samples which is thought to be a partial product of the Hsp70 gene. The sequencing results show that PCR amplification product is Hsp70 partial gene with a nucleotide variation in the 65^th base which has no effect on amino acid changes. The results indicate that Hsp70 gene is expressed in Emilia sonchifolia and Sphagneticola trilobata grown in UI and KRC.