FT-IR Transflection Micro-Spectroscopy Study on Normal Human Breast Cells after Exposure to a Proton Beam
Open Access
- 7 January 2021
- journal article
- research article
- Published by MDPI AG in Applied Sciences
- Vol. 11 (2), 540
- https://doi.org/10.3390/app11020540
Abstract
Fourier transform infrared micro-spectroscopy (μ-FT-IR) is nowadays considered a valuable tool for investigating the changes occurring in human cells after exposure to ionizing radiation. Recently, considerable attention has been devoted to the use of this optical technique in the study of cells exposed to proton beams, that are being increasingly adopted in cancer therapy. Different experimental configurations are used for proton irradiation and subsequent spectra acquisition. To facilitate the use of μ-FT-IR, it may be useful to investigate new experimental approaches capable of speeding up and simplifying the irradiation and measurements phases. Here, we propose the use of low-e-substrates slides for cell culture, allowing the irradiation and spectra acquisition in transflection mode in a fast and direct way. In recent years, there has been a wide debate about the validity of these supports, but many researchers agree that the artifacts due to the presence of the electromagnetic standing wave effects are negligible in many practical cases. We investigated human normal breast cells (MCF-10 cell line) fixed immediately after the irradiation with graded proton radiation doses (0, 0.5, 2, and 4 Gy). The spectra obtained in transflection geometry showed characteristics very similar to those present in the spectra acquired in transmission geometry and confirm the validity of the chosen approach. The analysis of spectra indicates the occurrence of significant changes in DNA and lipids components of cells. Modifications in protein secondary structure are also evidenced.This publication has 54 references indexed in Scilit:
- Identification of Early Biomarkers during Acetaminophen-Induced Hepatotoxicity by Fourier Transform Infrared MicrospectroscopyPLOS ONE, 2012
- Spectral pre-processing for biomedical vibrational spectroscopy and microspectroscopic imagingChemometrics and Intelligent Laboratory Systems, 2012
- Fourier Transform Infrared Microspectroscopy and Multivariate Methods for Radiobiological DosimetryRadiation Research, 2010
- Diagnosis of Cell Death by Means of Infrared SpectroscopyBiophysical Journal, 2009
- Infrared spectroscopy of proteinsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 2007
- Biochemical Alterations in Human Cells Irradiated with α Particles Delivered by Macro- or MicrobeamsRadiation Research, 2007
- Infrared microspectroscopy study of γ-irradiated and H2O2-treated human cellsInternational Journal of Radiation Biology, 2005
- FTIR spectroscopy studies of nucleic acid damageTalanta, 2000
- Apoptosis is associated with an increase in saturated fatty acid containing phospholipids in the neuronal cell line, HN2–5Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1996
- Radiation-induced structural modifications in dsDNA analysed by FT-Raman spectroscopyInternational Journal of Radiation Biology, 1996