Activation of the G‐Protein‐Coupled Receptor Rhodopsin by Water
- 28 June 2020
- journal article
- research article
- Published by Wiley in Angewandte Chemie
- Vol. 60 (5), 2288-2295
- https://doi.org/10.1002/anie.202003342
Abstract
Visual rhodopsin is an important archetype for G‐protein–coupled receptors, which are membrane proteins implicated in cellular signal transduction. Here we show experimentally for the first time that ~80 water molecules flood rhodopsin upon light absorption to form a solvent‐swollen active state. An influx of mobile water is sufficient for activating the photoreceptor, and is supported by molecular dynamics (MD) simulations. Combined force‐based measurements involving osmotic and hydrostatic pressure indicate the expansion occurs by changes in cavity volumes, together with greater hydration in the active metarhodopsin‐II state. Moreover, we discovered that binding and release of the C‐terminal helix of transducin is coupled to hydration changes as may occur in visual signal amplification. Hydration–dehydration explains signaling by a dynamic allosteric mechanism, in which the soft membrane matter (lipids and water) has a pivotal role in the catalytic G‐protein cycle.Funding Information
- National Science Foundation of Sri Lanka (CHE 1904125, MCB 1817862)
- National Institutes of Health (EY012049, EY026041, EY014614)
- Russian Foundation for Fundamental Investigations (16-04-00494A)
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