Attenuation of Hyperoxic Lung Injury in Newborn Thioredoxin-1-Overexpressing Mice through the Suppression of Proinflammatory Cytokine mRNA Expression
Open Access
- 20 March 2020
- journal article
- research article
- Published by MDPI AG in Biomedicines
- Vol. 8 (3), 66
- https://doi.org/10.3390/biomedicines8030066
Abstract
The role of thioredoxin-1 (TRX), a small redox-active protein with antioxidant effects, during hyperoxic lung injury in newborns remains undetermined. We investigated TRX impact on hyperoxic lung injury in newborn TRX transgenic (TRX-Tg) and wildtype (WT) mice exposed to 21% or 95% O2 for four days, after which some mice were allowed to recover in room air for up to 14 days. Lung morphology was assessed by hematoxylin/eosin and elastin staining, as well as immunostaining for macrophages. The gene expression levels of proinflammatory cytokines were evaluated using quantitative real-time polymerase chain reaction. During recovery from hyperoxia, TRX-Tg mice exhibited an improved mean linear intercept length and increased number of secondary septa in lungs compared with the WT mice. Neonatal hyperoxia enhanced the mRNA expression levels of proinflammatory cytokines in the lungs of both TRX-Tg and WT mice. However, interleukin-6, monocyte chemoattractant protein-1, and chemokine (C-X-C motif) ligand 2 mRNA expression levels were reduced in the lungs of TRX-Tg mice compared with the WT mice during recovery from hyperoxia. Furthermore, TRX-Tg mice exhibited reduced macrophage infiltration in lungs during recovery. These results suggest that in newborn mice TRX ameliorates hyperoxic lung injury during recovery likely through the suppression of proinflammatory cytokines.This publication has 43 references indexed in Scilit:
- Thioredoxin-1 Protects against Neutrophilic Inflammation and Emphysema Progression in a Mouse Model of Chronic Obstructive Pulmonary Disease ExacerbationPLOS ONE, 2013
- Thioredoxin Ameliorates Cutaneous Inflammation by Regulating the Epithelial Production and Release of Pro-Inflammatory CytokinesFrontiers in Immunology, 2013
- Silencing hyperoxia-induced C/EBPα in neonatal mice improves lung architecture via enhanced proliferation of alveolar epithelial cellsAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2011
- Long term consequences of oxygen therapy in the neonatal periodSeminars in Fetal and Neonatal Medicine, 2010
- Inflammation and cancer: macrophage migration inhibitory factor (MIF)--the potential missing linkQJM: An International Journal of Medicine, 2010
- Thioredoxin-1 Ameliorates Cigarette Smoke-Induced Lung Inflammation and Emphysema in MiceThe Journal of pharmacology and experimental therapeutics, 2008
- Thioredoxin prevents the development and progression of elastase-induced emphysemaBiochemical and Biophysical Research Communications, 2007
- Overexpression of Redox-Active Protein Thioredoxin-1 Prevents Development of Chronic Pancreatitis in MiceAntioxidants and Redox Signaling, 2006
- Extracellular thioredoxin levels are increased in patients with acute lung injuryThorax, 2006
- Protective roles of redox-active protein thioredoxin-1 for severe acute pancreatitisAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2006