FAM3B/PANDER-Like Carbohydrate-Binding Domain in a Glycosyltransferase, POMGNT1
- 19 April 2020
- book chapter
- protocol
- Published by Springer Science and Business Media LLC
- Vol. 2132, 609-619
- https://doi.org/10.1007/978-1-0716-0430-4_52
Abstract
Protein O-mannose β1,2-N-acetylglucosaminyltransferase 1 (POMGNT1) is one of the gene products responsible for α-dystroglycanopathy, which is a type of congenital muscular dystrophy caused by O-mannosyl glycan defects. The originally identified function of POMGNT1 was as a glycosyltransferase that catalyzes the formation of the GlcNAcβ1-2Man linkage of O-mannosyl glycan, but the enzyme function is not essential for α-dystroglycanopathy pathogenesis. Our recent study revealed that the stem domain of POMGNT1 has a carbohydrate-binding ability, which recognizes the GalNAcβ1-3GlcNAc structure. This carbohydrate-binding activity is required for the formation of the ribitol phosphate (RboP)-3GalNAcβ1-3GlcNAc structure by fukutin. This protocol describes methods to assess the carbohydrate-binding activity of the POMGNT1 stem domain.Keywords
This publication has 15 references indexed in Scilit:
- Glycosylation with ribitol-phosphate in mammals: New insights into the O -mannosyl glycanBiochimica et Biophysica Acta (BBA) - General Subjects, 2017
- The Muscular Dystrophy Gene TMEM5 Encodes a Ribitol β1,4-Xylosyltransferase Required for the Functional Glycosylation of DystroglycanOnline Journal of Public Health Informatics, 2016
- Carbohydrate-binding domain of the POMGnT1 stem region modulates O -mannosylation sites of α-dystroglycanProceedings of the National Academy of Sciences of the United States of America, 2016
- Identification of a Post-translational Modification with Ribitol-Phosphate and Its Defect in Muscular DystrophyCell Reports, 2016
- PANcreatic-DERived factor: Novel hormone PANDERing to glucose regulationFEBS Letters, 2011
- O -Mannosyl Phosphorylation of Alpha-Dystroglycan Is Required for Laminin BindingScience, 2010
- Molecular interaction between fukutin and POMGnT1 in the glycosylation pathway of α-dystroglycanBiochemical and Biophysical Research Communications, 2006
- Structure–function analysis of human protein O-linked mannose β1,2-N-acetylglucosaminyltransferase 1, POMGnT1Biochemical and Biophysical Research Communications, 2004
- Post-translational disruption of dystroglycan–ligand interactions in congenital muscular dystrophiesNature, 2002
- Muscular Dystrophy and Neuronal Migration Disorder Caused by Mutations in a Glycosyltransferase, POMGnT1Developmental Cell, 2001