Evaluation of Allicin Against Alveolar Echinococcosis In Vitro and in a Mouse Model
Open Access
- 18 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Acta Parasitologica
- Vol. 67 (1), 79-93
- https://doi.org/10.1007/s11686-021-00434-z
Abstract
Purpose At present, the chemotherapy for alveolar echinococcosis (AE) is mainly based on albendazole (ABZ). However, more than 20% of patients fail chemotherapy. Therefore, new and more effective treatments are urgently needed. Allicin has been reported to have antibacterial and antiparasitic effects. The objectives of the present study were to investigate the in vivo and in vitro efficacy of allicin against Echinococcus multilocularis (E. multilocularis). Methods The effects of allicin on protoscolex survival and structural changes were evaluated in vitro. The 4-week-old BALB/c male mice used for in vivo modelling underwent inoculation of E. multilocularis protoscoleces by intraperitoneal injection, followed by intragastric administration of allicin for 6 weeks. Then, the effects of allicin on lymphocyte subsets, metacestode growth and host tissue matrix metalloproteinase 2 (MMP2)/MMP9 expression around metacestodes in mice were evaluated. The toxicity of allicin was further evaluated in vivo and in vitro. Results Att 40 μg/mL, allicin showed a killing effect on protoscoleces in vitro and treatment resulted in the destruction of protoscolex structure. Molecular docking showed that allicin could form hydrogen bonds with E. multilocularis cysteine enzymes. After 6 weeks of in vivo allicin treatment, the spleen index of mice was increased and the weight of metacestodes was reduced. Allicin increased the proportion of CD4+ T cells and decreased the proportion of CD8+ T cells in the peripheral blood and spleen. Pathological analysis of the metacestodes showed structural disruption of the germinal and laminated layers after allicin treatment. In addition, allicin inhibited the expression of MMP2 and MMP9 in metacestode-surrounding host tissues. At 160 μg/mL, allicin had no significant toxicity to normal hepatocytes but could inhibit hepatoma cell proliferation. At 30 mg/kg, allicin had no significant hepatorenal toxicity in vivo. Conclusion These results suggest that allicin exerts anti-E. multilocularis effects in vitro and in vivo and can enhance immune function in mice, with the potential to be developed as a lead compound against echinococcosis.Keywords
Funding Information
- Natural Science Foundation of Jilin Province (2020-ZJ-956Q)
- the National key R & D projects (2017YFC0909900)
This publication has 45 references indexed in Scilit:
- Allicin enhances host pro-inflammatory immune responses and protects against acute murine malaria infectionMalaria Journal, 2012
- Understanding the laminated layer of larval Echinococcus II: immunologyTrends in Parasitology, 2011
- Allicin and derivates are cysteine protease inhibitors with antiparasitic activityBioorganic & Medicinal Chemistry Letters, 2010
- Triggering and modulation of the host-parasite interplay byEchinococcus multilocularis: a reviewParasitology, 2009
- Garlic: Nature's Protection Against Physiological ThreatsCritical Reviews in Food Science and Nutrition, 2009
- Systemic Production of IFN-αby Garlic (Allium Sativum) in HumansJournal of Interferon & Cytokine Research, 2007
- Antimalarial Activity of Allicin, a Biologically Active Compound from Garlic ClovesAntimicrobial Agents and Chemotherapy, 2006
- Plant active components – a resource for antiparasitic agents?Trends in Parasitology, 2005
- Long-term administration of aqueous garlic extract (AGE) alleviates liver fibrosis and oxidative damage induced by biliary obstruction in ratsLife Sciences, 2005
- Increased Activation and Oligoclonality of Peripheral CD8+T Cells in the Chronic Human Helminth Infection Alveolar EchinococcosisInfection and Immunity, 2002