Semen Modulates Inflammation and Angiogenesis in the Reproductive Tract of Female Rabbits
Open Access
- 24 November 2020
- Vol. 10 (12), 2207
- https://doi.org/10.3390/ani10122207
Abstract
The maternal environment modulates immune responses to facilitate embryo development and ensure pregnancy. Unraveling this modulation could improve the livestock breeding systems. Here it is hypothesized that the exposure of the female rabbit reproductive tract to semen, as well as to early embryos, modulates inflammation and angiogenesis among different tissue segments. qPCR analysis of the gene expression changes of the anti-inflammatory interleukin-10 (IL10) and transforming growth factor beta family (TGFβ1–3) and the angiogenesis mediator vascular endothelial growth factor (VEGF-A) were examined in response to mating or insemination with sperm-free seminal plasma (SP). Reproductive tract segment (cervix to infundibulum) samples were obtained in Experiment 1, 20 h after gonadotropin-releasing hormone (GnRH) stimulation (control), natural mating (NM) or vaginal infusion with sperm-free SP (SP-AI). Additionally, segmented samples were also obtained at 10, 24, 36, 68 or 72 h after GnRH-stimulation and natural mating (Experiment 2). The results of gene expression, analyzed by quantitative PCR, showed that NM effects were mainly localized in the uterine tissues, depicting clear temporal variation, while SP-AI effects were restricted to the oviduct. Changes in anti-inflammatory and angiogenesis mediators indicate an early response in the uterus and a late modulation in the oviduct either induced by semen or preimplantation embryos. This knowledge could be used in the implementation of physiological strategies in breeding systems to face the new challenges on rabbit productivity and sustainability.Keywords
This publication has 50 references indexed in Scilit:
- Regulation of Angiogenesis in the Primate Endometrium: Vascular Endothelial Growth FactorSeminars in Reproductive Medicine, 2009
- Simultaneous Inference in General Parametric ModelsBiometrical Journal, 2008
- Regulation of the VEGF-System in the Endometrium during Steroid-Replacement and Early Pregnancy of PigsExperimental and Clinical Endocrinology & Diabetes, 2003
- Role of vascular endothelial growth factor in endometrial vascular events before implantation in ratsReproduction, 2001
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Neuroendocrine Regulation of GnRH Release in Induced OvulatorsFrontiers in Neuroendocrinology, 2000
- TGF-β SIGNAL TRANSDUCTIONAnnual Review of Biochemistry, 1998
- Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones.The Journal of Experimental Medicine, 1989
- Sperm Transport in the Reproductive Tract of the Female Rabbit: II. The Sustained Phase of Transport1Biology of Reproduction, 1978
- Phagocytosis of spermatozoa by the rabbit vaginaThe Anatomical Record, 1977