Detection of Active Granzyme A in NK92 Cells with Fluorescent Activity-Based Probe
Open Access
- 6 March 2020
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Medicinal Chemistry
- Vol. 63 (6), 3359-3369
- https://doi.org/10.1021/acs.jmedchem.9b02042
Abstract
Cytotoxic T-lymphocytes (CTLs) and natural killer cells (NKs) kill compromised cells to defend against tumor and viral infections. Both effector cell types use multiple strategies to induce target cell death including Fas/CD95 activation; and the release of perforin and a group of lymphocyte granule serine proteases called granzymes. Granzymes have relatively broad and overlapping substrate specificities and may hydrolyze a wide range of peptidic epitopes; it is therefore challenging to identify their natural and synthetic substrates and to distinguish their localization and functions. Here, we present a specific and potent substrate, an inhibitor, and an activity-based probe of Granzyme A (GrA) that can be used to follow functional GrA in cells.Funding Information
- Ministerstwo Nauki i Szkolnictwa Wyższego
- Fundacja na rzecz Nauki Polskiej (2016-3/24)
- L'Or?al Polska
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