Glypican-1 Level Is Elevated in Extracellular Vesicles Released from MC38 Colon Adenocarcinoma Cells Overexpressing Snail
Open Access
- 29 June 2020
- Vol. 9 (7), 1585
- https://doi.org/10.3390/cells9071585
Abstract
The transcription factor Snail triggers epithelial-to-mesenchymal transition (EMT), endowing cancer cells with invasive properties during tumor progression. Extracellular vesicles (EVs) released from cancer cells at various stages of cancer progression are known to influence the tumor pre-metastatic niche and metastatic potential. The aim of this study was to analyze the effect of Snail on murine colon adenocarcinoma cells (MC38 line) and on the characteristics of their EVs. Stable clones of Snail-overexpressing MC38 cells were investigated in vitro versus Mock cells. Increased expression of matrix metalloproteinase MMP-14 and augmented activity of MMP-9 and -14 were observed in Snail-MC38 cells. There was no change in the transcriptomic profile of proteoglycans in Snail-MC38 cells; however, the protein level of Glypican-1 (GPC1) was enhanced in EVs released from those cells. Our finding that GPC1 protein level was enhanced in EVs released from MC38 cells that overexpressed Snail and were in an early EMT stage might explain the specificity of the GPC1 biomarker in colon cancer diagnosis. Further, our data suggest that Snail, by changing the level of GPC1 on EVs released by colon cancer cells, may affect the generation of a distant premetastatic niche and metastatic organotropism in colon adenocarcinoma.Funding Information
- Narodowe Centrum Nauki (2011/02/A/NZ3/00068; 2018/29/B/NZ5/01756)
- Ligue Contre le Cancer (30036506-UMR7369)
This publication has 42 references indexed in Scilit:
- Regulation of miRNAs by Snail during epithelial-to-mesenchymal transition in HT29 colon cancer cellsScientific Reports, 2019
- EMT: Present and future in clinical oncologyMolecular Oncology, 2017
- Neuromedin U is upregulated by Snail at early stages of EMT in HT29 colon cancer cellsBiochimica et Biophysica Acta (BBA) - General Subjects, 2016
- EMT: 2016Cell, 2016
- Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012International Journal of Cancer, 2014
- Epithelial-to-Mesenchymal Transition in Pancreatic AdenocarcinomaThe Scientific World Journal, 2010
- Transcriptional regulation of cell polarity in EMT and cancerOncogene, 2008
- Epithelial-Mesenchymal Transition: At the Crossroads of Development and Tumor MetastasisDevelopmental Cell, 2008
- Complex networks orchestrate epithelial–mesenchymal transitionsNature Reviews Molecular Cell Biology, 2006
- Epithelial–mesenchymal transitions in tumour progressionNature Reviews Cancer, 2002