Epigenetic regulator Stuxnet modulates octopamine effect on sleep through a Stuxnet‐Polycomb‐Octβ2R cascade
- 7 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in EMBO Reports
- Vol. 22 (2), e47910
- https://doi.org/10.15252/embr.201947910
Abstract
Sleep homeostasis is crucial for sleep regulation. The role of epigenetic regulation in sleep homeostasis is unestablished. Previous studies showed that octopamine is important for sleep homeostasis. However, the regulatory mechanism of octopamine reception in sleep is unknown. In this study, we identify an epigenetic regulatory cascade (Stuxnet‐Polycomb‐Octβ2R) that modulates the octopamine receptor in Drosophila. We demonstrate that stuxnet positively regulates Octβ2R through repression of Polycomb in the ellipsoid body of the adult fly brain and that Octβ2R is one of the major receptors mediating octopamine function in sleep homeostasis. In response to octopamine, Octβ2R transcription is inhibited as a result of stuxnet downregulation. This feedback through the Stuxnet‐Polycomb‐Octβ2R cascade is crucial for sleep homeostasis regulation. This study demonstrates a Stuxnet‐Polycomb‐Octβ2R‐mediated epigenetic regulatory mechanism for octopamine reception, thus providing an example of epigenetic regulation of sleep homeostasis.Keywords
Funding Information
- National Natural Science Foundation of China (31772535, 31730076, 31830058)
This publication has 46 references indexed in Scilit:
- Cell-Type-Specific Profiling of Gene Expression and Chromatin Binding without Cell Isolation: Assaying RNA Pol II Occupancy in Neural Stem CellsDevelopmental Cell, 2013
- Histone methyltransferase MLL3 contributes to genome-scale circadian transcriptionProceedings of the National Academy of Sciences of the United States of America, 2013
- Genetically encoded dendritic marker sheds light on neuronal connectivity inDrosophilaProceedings of the National Academy of Sciences of the United States of America, 2010
- Identification of a Neural Circuit that Underlies the Effects of Octopamine on Sleep:Wake BehaviorNeuron, 2010
- Octopamine Regulates Sleep inDrosophilathrough Protein Kinase A-Dependent MechanismsJournal of Neuroscience, 2008
- The NAD+-Dependent Deacetylase SIRT1 Modulates CLOCK-Mediated Chromatin Remodeling and Circadian ControlCell, 2008
- Octopamine and tyramine influence the behavioral profile of locomotor activity in the honey bee (Apis mellifera)Journal of Insect Physiology, 2006
- Circadian Regulator CLOCK Is a Histone AcetyltransferaseCell, 2006
- Spatiotemporal Rescue of Memory Dysfunction in DrosophilaScience, 2003
- Living synaptic vesicle marker: Synaptotagmin‐GFPgenesis, 2002