Macroautophagy occurs in distal TMV-uninfected root tip tissue of tomato taking place systemic PCD
- 27 May 2017
- journal article
- research article
- Published by Springer Science and Business Media LLC in Protoplasma
- Vol. 255 (1), 3-9
- https://doi.org/10.1007/s00709-017-1125-9
Abstract
Autophagy is an important mechanism for recycling cell materials upon encountering stress conditions. Our previous studies had shown that TMV infection could lead to systemic PCD in the distal uninfected tissues, including root tip and shoot tip tissues. But it is not clear whether there is autophagy in the distal apical meristem of TMV-induced plants. To better understand the autophagy process during systemic PCD, here we investigated the formation and type of autophagy in the root meristem cells occurring PCD. Transmission electron microscopy assay revealed that the autophagic structures formed by the fusion of vesicles, containing the sequestered cytoplasm, multilamellar bodies, and degraded mitochondria. In the PCD progress, many mitochondria appeared degradation with blurred inner membrane structure. And the endoplasmic reticulum was broke into small fragments. Finally, the damaged mitochodria were engulfed and degraded by the autophagosomes. These results indicated that during the systemic PCD process of root tip cells, the classical macroautophagy occurred, and the cell contents and damaged organelles (mitochondria) would be self-digested by autophagy.Keywords
Funding Information
- Natural Science Foundation of Shanghai (CN) (15ZR1416700)
This publication has 40 references indexed in Scilit:
- Delphinidin Induces Autolysosome as well as Autophagosome Formation and Delphinidin‐Induced Autophagy Exerts a Cell Protective RoleJournal of Biochemical and Molecular Toxicology, 2012
- Autophagy: Pathways for Self-Eating in Plant CellsAnnual Review of Plant Biology, 2012
- Mitochondrial quality control by the ubiquitin–proteasome systemBiochemical Society Transactions, 2011
- Variations on a theme: plant autophagy in comparison to yeast and mammalsProtoplasma, 2011
- Spinster is required for autophagic lysosome reformation and mTOR reactivation following starvationProceedings of the National Academy of Sciences of the United States of America, 2011
- Degradation of Oxidized Proteins by Autophagy during Oxidative Stress in ArabidopsisPlant Physiology, 2006
- The PET1-CMS Mitochondrial Mutation in Sunflower Is Associated with Premature Programmed Cell Death and Cytochrome c ReleaseTHE PLANT CELL ONLINE, 2001
- Programmed cell death, mitochondria and the plant hypersensitive responseNature, 2001
- Plant VacuolesTHE PLANT CELL ONLINE, 1999
- Analog modeling of glucagon-induced autophagy in rat liver: I. Conceptual and mathematical model of telolysosome-autophagosome-autolysosome interactionExperimental Cell Research, 1975