PENGARUH SEREAL BERBAHAN SAGU DAN Moringa oleifera TERHADAP KADAR GLUKOSA DARAH TIKUS YANG DIINDUKSI ALOKSAN - IN PRESS -

Abstract

The Effects of Cereal Made From Sagu and Moringa oleifera on the Blood Glucose Level of Alloxan-Induced RatsDiabetes Mellitus (DM) type 2 could increase oxidative stress and blood glucose level. Resistant starch compounds in Cersa Mori have an antidiabetic properties.This research aimed to analyze the effect of Cersa Mori on fasting blood glucose (FBG) level of diabetic white rats induced by alloxan. This is a true experimental study with a randomized pre-post control group design using 27 male Wistar strain rats divided into 3 groups randomly, i.e (KN) feed and aquades, (KP) glibenclamide 0.126mg/200gBB/day, (P) Cersa Mori 5g/200gBB/day. KP and P groups were given alloxan 125 mg/KgBB subcutaneously and the intervention was carried out for 30 days. FBG level was measured using the GOD-PAP method. The results of Paired T-Test showed the effect of Cersa Mori on lowering FBG level in hyperglycemic rats (P=0,006). One-Way ANOVA test showed that Cersa Mori reduced FBG level, which was equalent to those given glibenclamide (P=0,366). It can be concluded that giving Cersa Mori 5g/200gBB/day for 30 days had a significant effect on lowering FBG level. Keywords: alloxan; Cersa Mori; diabetic rats; fasting blood glucose level; resistant strachABSTRAKDiabetes Mellitus (DM) tipe 2 dapat memicu stres oksidatif dan meningkatkan kadar glukosa darah puasa (GDP). Senyawa pati resisten dalam sereal siap saji Cersa Mori memiliki sifat antidiabetik. Penelitian ini bertujuan menganalisis pengaruh pemberian Cersa Mori terhadap kadar glukosa darah tikus putih diabetes yang diinduksi aloksan. Penelitian true-experimental ini menggunakan randomized pre-post control group design. Sampel sebanyak 27 ekor tikus jantan galur Wistar dibagi menjadi 3 kelompok secara acak yaitu; (KN) pakan dan akuades, (KP) glibenklamid 0,126 mg/200gBB/hari, (P) Cersa Mori 5g/200gBB/hari. KP sampai P diberikan aloksan 125 mg/KgBB secara subkutan dan intervensi dilakukan selama 30 hari. Pengukuran GDP menggunakan metode GOD-PAP. Hasil Uji-T menunjukkan pengaruh Cersa Mori dalam menurunkan GDP tikus hiperglikemia (P=0,006). Uji ANOVA satu arah menunjukkan penurunan GDP pada kelompok Cersa Mori (P) setara dengan tikus yang diberi glibenklamid (P=0,366). Dengan demikian dapat disimpulkan bahwa pemberian Cersa Mori dosis 5g/200gBB/hari selama 30 hari berpengaruh terhadap penurunan GDP secara signifikan.