m6A mRNA Methylation Controls Functional Maturation in Neonatal Murine β-Cells
- 1 August 2020
- journal article
- research article
- Published by American Diabetes Association in Diabetes
- Vol. 69 (8), 1708-1722
- https://doi.org/10.2337/db19-0906
Abstract
TheN(6)-methyladenosine (m(6)A) RNA modification is essential during embryonic development of various organs. However, its role in embryonic and early postnatal islet development remains unknown. Mice in which RNA methyltransferase-like 3/14 (Mettl3/14) were deleted in Ngn3(+)endocrine progenitors (Mettl3/14(nKO)) developed hyperglycemia and hypoinsulinemia at 2 weeks after birth. We found that Mettl3/14 specifically regulated both functional maturation and mass expansion of neonatal beta-cells before weaning. Transcriptome and m(6)A methylome analyses provided m(6)A-dependent mechanisms in regulating cell identity, insulin secretion, and proliferation in neonatal beta-cells. Importantly, we found that Mettl3/14 were dispensable for beta-cell differentiation but directly regulated essential transcription factor MafA expression at least partially via modulating its mRNA stability. Failure to maintain this modification impacted the ability to fulfill beta-cell functional maturity. In both diabeticdb/dbmice and patients with type 2 diabetes (T2D), decreased Mettl3/14 expression in beta-cells was observed, suggesting its possible role in T2D. Our study unraveled the essential role of Mettl3/14 in neonatal beta-cell development and functional maturation, both of which determined functional beta-cell mass and glycemic control in adulthood.Funding Information
- National Natural Sciences Foundation of China (81670700, 81870527)
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