Influence of gastrectomy for gastric cancer treatment on faecal microbiome and metabolome profiles
Open Access
- 16 January 2020
- Vol. 69 (8), 1404-1415
- https://doi.org/10.1136/gutjnl-2019-319188
Abstract
Objective Recent evidence points to the gut microbiome’s involvement in postoperative outcomes, including after gastrectomy. Here, we investigated the influence of gastrectomy for gastric cancer on the gut microbiome and metabolome, and how it related to postgastrectomy conditions. Design We performed shotgun metagenomics sequencing and capillary electrophoresis time-of-flight mass spectrometry-based metabolomics analyses on faecal samples collected from participants with a history of gastrectomy for gastric cancer (n=50) and compared them with control participants (n=56). Results The gut microbiota in the gastrectomy group showed higher species diversity and richness (pConclusion Our results reveal alterations of gut microbiota after gastrectomy, suggesting its association with postoperative comorbidities. The multi-omic approach applied in this study could complement the follow-up of patients after gastrectomy.Keywords
Funding Information
- Takeda Science Foundation
- Suzuken Memorial Foundation
- Joint Research Project of the Institute of Medical Science, the University of Tokyo
- Precursory Research for Embryonic Science and Technology (JPMJPR1537, JPMJPRI507)
- National Cancer Center Research and Development Fund (25-A-4, 28-A-4, 29-A-13, 29-A-6)
- Japan Science and Technology Agency-AIP Acceleration Research (JPMJCR19U3)
- Exploratory Research for Advanced Technology (JPMJER1902)
- Food Science Institute Foundation
- Program for the Advancement of Research in Core Projects under Keio University’s Longevity Initiative
- Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University
- Japan Agency for Medical Research and Development (JP18ek0109187, JP19cm0106464, JP19gm1010009)
- Yasuda Memorial Medical Foundation
- Japan Society for the Promotion of Science-KAKENHI (JPMJCR19U3)
This publication has 54 references indexed in Scilit:
- Richness of human gut microbiome correlates with metabolic markersNature, 2013
- Predictive functional profiling of microbial communities using 16S rRNA marker gene sequencesNature Biotechnology, 2013
- Inferring Correlation Networks from Genomic Survey DataPLoS Computational Biology, 2012
- Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatmentGenome Biology, 2012
- Metagenomic biomarker discovery and explanationGenome Biology, 2011
- The Human Oral Microbiome Database: a web accessible resource for investigating oral microbe taxonomic and genomic informationDatabase: The Journal of Biological Databases and Curation, 2010
- Human gut microbiota in obesity and after gastric bypassProceedings of the National Academy of Sciences of the United States of America, 2009
- Metabolite profiling of human colon carcinoma – deregulation of TCA cycle and amino acid turnoverMolecular Cancer, 2008
- Surgery for morbid obesityHeart, 2007
- KEGG: Kyoto Encyclopedia of Genes and GenomesNucleic Acids Research, 2000