A rapid method for quantification of persistent and mobile organic substances in water using supercritical fluid chromatography coupled to high-resolution mass spectrometry
Open Access
- 1 August 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 412 (20), 4941-4952
- https://doi.org/10.1007/s00216-020-02722-5
Abstract
Persistent and mobile organic substances (PM substances) are a threat to the quality of our water resources. While screening studies revealed widespread occurrence of many PM substances, rapid trace analytical methods for their quantification in large sample sets are missing. We developed a quick and generic analytical method for highly mobile analytes in surface water, groundwater, and drinking water samples based on enrichment through azeotrope evaporation (4 mL water and 21 mL acetonitrile), supercritical fluid chromatography (SFC) coupled to high-resolution mass spectrometry (HRMS), and quantification using a compound-specific correction factor for apparent recovery. The method was validated using 17 PM substances. Sample preparation recoveries were between 60 and 110% for the vast majority of PM substances. Strong matrix effects (most commonly suppressive) were observed, necessitating a correction for apparent recoveries in quantification. Apparent recoveries were neither concentration dependent nor dependent on the water matrix (surface or drinking water). Method detection and quantification limits were in the single- to double-digit ng L(-1)ranges, precision expressed as relative standard deviation of quadruplicate quantifications was on average < 10%, and trueness experiments showed quantitative results within +/- 30% of the theoretical value in 77% of quantifications. Application of the method to surface water, groundwater, raw water, and finished drinking water revealed the presence of acesulfame and trifluoromethanesulfonic acid up to 70 and 19 mu g L-1, respectively. Melamine, diphenylguanidine,p-dimethylbenzenesulfonic acid, and 4-hydroxy-1-(2-hydroxyethyl)-2,2,6,6-tetramethylpiperidine were found in high ng L(-1)concentrations.Keywords
Funding Information
- German BMBF in the frame of the collaborative international consortium WATERJPI2013 – PROMOTE of the Water Challenges for a Changing World Joint Programming Initiative (Water JPI) Pilot Call (02WU1347A)
This publication has 31 references indexed in Scilit:
- Emerging contaminants (pharmaceuticals, personal care products, a food additive and pesticides) in waters of Sydney estuary, AustraliaMarine Pollution Bulletin, 2015
- Analytical method development for the determination of emerging contaminants in water using supercritical-fluid chromatography coupled with diode-array detectionAnalytical and Bioanalytical Chemistry, 2015
- Quantification of four artificial sweeteners in Finnish surface waters with isotope-dilution mass spectrometryEnvironmental Pollution, 2014
- Impact of injection solvents on supercritical fluid chromatographyJournal of Chromatography A, 2013
- Simultaneous determination of PPCPs, EDCs, and artificial sweeteners in environmental water samples using a single-step SPE coupled with HPLC–MS/MS and isotope dilutionTalanta, 2013
- History of supercritical fluid chromatography: Instrumental developmentJournal of Bioscience and Bioengineering, 2013
- Retention mechanisms in super/subcritical fluid chromatography on packed columnsJournal of Chromatography A, 2009
- Supercritical fluid chromatography for the 21st centuryThe Journal of Supercritical Fluids, 2008
- Determination of sulfonic acids and alkylsulfates by ion chromatography in waterTalanta, 2008
- Characterization of Groundwater Samples from Superfund Sites by Gas Chromatography/Mass Spectrometry and Liquid Chromatography/Mass SpectrometryEnvironmental Science & Technology, 1996