Protein denature extracts of Lactobacillus crispatus KT‐11 strain promote interleukin 12p40 production via Toll‐like receptor 2 in J774.1 cell culture
- 27 December 2020
- journal article
- research article
- Published by Hindawi Limited in Journal of Food Biochemistry
- Vol. 45 (2), e13599
- https://doi.org/10.1111/jfbc.13599
Abstract
The objective of the present study is to investigate the mechanism and the cell components of Lactobacillus crispatus KT‐11 strain (KT‐11) that induce interleukin (IL)‐12p40 production. IL‐12p40 production induced by KT‐11 was decreased in the presence of inhibitors of extracellular signal‐regulated kinase or nuclear factor kappa B. Guanidine hydrochloride, urea or lithium chloride extract of KT‐11 induced IL‐12p40 production, but production was suppressed in the presence of Toll‐like receptor 2‐specific neutralizing antibody. These findings suggest that the protein denature extracts of KT‐11 promote IL‐12p40 production via Toll‐like receptor 2 in J774.1 cells. Practical applications Heat‐treated lactic acid bacteria are added to some foods because it is easier to store and transport, and have less interference with other food ingredients compared with living lactic acid bacteria. Heat‐treated Lactobacillus crispatus KT‐11 strain (KT‐11) is included in some foods because of good handling characteristics and good dispersibility in the food product. We have previously reported that the administration of KT‐11 led to beneficial health effects through the regulation of the immune system in mice, but the mechanism is not clear. We found that protein denature extracts, which may include proteins such as SLP and SLAPs, of KT‐11 cells promoted IL‐12p40 production via TLR2 in the J774.1 cell culture. This result will contribute to providing more effective lactic acid bacteria functional food.Keywords
This publication has 22 references indexed in Scilit:
- Nucleic acids of Enterococcus faecalis strain EC-12 are potent Toll-like receptor 7 and 9 ligands inducing interleukin-12 production from murine splenocytes and murine macrophage cell line J774.1FEMS Immunology & Medical Microbiology, 2010
- Requirement for NF-κB signalling in a mouse model of lung adenocarcinomaNature, 2009
- Heat-Treated Lactobacillus crispatus KT Strains Reduce Allergic Symptoms in MiceJournal of Agricultural and Food Chemistry, 2009
- S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functionsProceedings of the National Academy of Sciences of the United States of America, 2008
- TLR2 – promiscuous or specific? A critical re-evaluation of a receptor expressing apparent broad specificityImmunobiology, 2008
- Activation of Toll-Like Receptor 9 by DNA from Different Bacterial SpeciesInfection and Immunity, 2006
- Lactobacillus surface layers and their applicationsFEMS Microbiology Reviews, 2005
- Augmentation of TH-1 type response by immunoactive AT oligonucleotide from lactic acid bacteria via Toll-like receptor 9 signalingBiochemical and Biophysical Research Communications, 2005
- MEK Is a Key Modulator for TLR5-induced Interleukin-8 and MIP3α Gene Expression in Non-transformed Human Colonic Epithelial CellsPublished by Elsevier BV ,2004
- Lipoteichoic Acids fromLactobacillusStrains Elicit Strong Tumor Necrosis Factor Alpha-Inducing Activities in Macrophages through Toll-Like Receptor 2Clinical and Vaccine Immunology, 2003