N-glycosylation of the transient receptor potential melastatin 8 channel is altered in pancreatic cancer cells
Open Access
- 31 August 2017
- journal article
- research article
- Published by IOS Press in Tumor Biology
- Vol. 39 (10)
- https://doi.org/10.1177/1010428317720940
Abstract
Transient receptor potential melastatin 8 (TRPM8), a membrane ion channel, is activated by thermal and chemical stimuli. In pancreatic ductal adenocarcinoma, TRPM8 is required for cell migration, proliferation, and senescence and is associated with tumor size and pancreatic ductal adenocarcinoma stages. Although the underlying mechanisms of these processes have yet to be described, this cation-permeable channel has been proposed as an oncological target. In this study, the glycosylation status of the TRPM8 channel was shown to affect cell proliferation, cell migration, and calcium uptake. TRPM8 expressed in the membrane of the Panc-1 pancreatic tumoral cell line is non-glycosylated, whereas human embryonic kidney cells transfected with human TRPM8 overexpress a glycosylated protein. Moreover, our data suggest that Ca2+ uptake is modulated by the glycosylation status of the protein, thus affecting cell proliferation.Keywords
This publication has 24 references indexed in Scilit:
- TRPM8 Ion Channels as Potential Cancer Biomarker and Target in Pancreatic CancerPublished by Elsevier BV ,2016
- Roles of TRPM8 Ion Channels in Cancer: Proliferation, Survival, and InvasionCancers, 2015
- Characterization of Functional Transient Receptor Potential Melastatin 8 Channels in Human Pancreatic Ductal Adenocarcinoma CellsPancreas, 2014
- Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells InvasionCells, 2014
- Post-Translational Modifications of TRP ChannelsCells, 2014
- N-Glycosylation of TRPM8 Ion Channels Modulates Temperature Sensitivity of Cold Thermoreceptor NeuronsOnline Journal of Public Health Informatics, 2012
- Regulation of TRP channels by N-linked glycosylationSeminars in Cell & Developmental Biology, 2006
- Trafficking and Assembly of the Cold-sensitive TRPM8 ChannelOnline Journal of Public Health Informatics, 2006
- A TRP Channel that Senses Cold Stimuli and MentholCell, 2002
- Identification of a cold receptor reveals a general role for TRP channels in thermosensationNature, 2002