The Growth of 3T3 Fibroblasts on PHB, PLA and PHB/PLA Blend Films at Different Stages of Their Biodegradation In Vitro
Open Access
- 28 December 2020
- Vol. 13 (1), 108
- https://doi.org/10.3390/polym13010108
Abstract
Over the past century there was a significant development and extensive application of biodegradable and biocompatible polymers for their biomedical applications. This research investigates the dynamic change in properties of biodegradable polymers: poly(3-hydroxybutyrate (PHB), poly-l-lactide (PLA), and their 50:50 blend (PHB/PLA)) during their hydrolytic non-enzymatic (in phosphate buffered saline (PBS), at pH = 7.4, 37 °C) and enzymatic degradation (in PBS supplemented with 0.25 mg/mL pancreatic lipase). 3T3 fibroblast proliferation on the polymer films experiencing different degradation durations was also studied. Enzymatic degradation significantly accelerated the degradation rate of polymers compared to non-enzymatic hydrolytic degradation, whereas the seeding of 3T3 cells on the polymer films accelerated only the PLA molecular weight loss. Surprisingly, the immiscible nature of PHB/PLA blend (showed by differential scanning calorimetry) led to a slower and more uniform enzymatic degradation in comparison with pure polymers, PHB and PLA, which displayed a two-stage degradation process. PHB/PLA blend also displayed relatively stable cell viability on films upon exposure to degradation of different durations, which was associated with the uneven distribution of cells on polymer films. Thus, the obtained data are of great benefit for designing biodegradable scaffolds based on polymer blends for tissue engineering.Keywords
Funding Information
- Russian Science Foundation (20-64-47008)
This publication has 47 references indexed in Scilit:
- Polyhydroxyalkanoates (PHA) for therapeutic applicationsMaterials Science and Engineering: C, 2018
- Biosynthesis of poly(3-hydroxybutyrate) copolymers by Azotobacter chroococcum 7B: A precursor feeding strategyPreparative Biochemistry & Biotechnology, 2016
- Bacterial polyhydroxyalkanoates: Still fabulous?Microbiological Research, 2016
- Bacterial-derived biopolymers: Advanced natural nanomaterials for drug delivery and tissue engineeringTrAC Trends in Analytical Chemistry, 2016
- Cell growth and accumulation of polyhydroxyalkanoates from CO 2 and H 2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646Bioresource Technology, 2013
- Bacterial polymers: biosynthesis, modifications and applicationsNature Reviews Microbiology, 2010
- Poly(lactic acid) modificationsProgress in Polymer Science, 2010
- Recent advances in microbial polyhydroxyalkanoatesProcess Biochemistry, 2005
- Bacterial Polyesters: Biosynthesis, Biodegradable Plastics and BiotechnologyBiomacromolecules, 2004
- Ring-opening polymerization of 3(S)-[(benzyloxycarbonyl)methyl]-1,4-dioxane-2,5-dione: a new route to a poly(.alpha.-hydroxy acid) with pendant carboxyl groupsMacromolecules, 1988