Induction of Native c-di-GMP Phosphodiesterases Leads to Dispersal of Pseudomonas aeruginosa Biofilms
- 18 March 2021
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 65 (4)
- https://doi.org/10.1128/aac.02431-20
Abstract
A decade of research has shown that the molecule c-di-GMP functions as a central second messenger in many bacteria. A high level of c-di-GMP is associated with biofilm formation whereas a low level of c-di-GMP is associated with a planktonic single-cell bacterial lifestyle. C-di-GMP is formed by diguanylate cyclases and is degraded by specific phosphodiesterases. We have previously presented evidence that ectopic expression in Pseudomonas aeruginosa of the Escherichia coli phosphodiesterase YhjH results in biofilm dispersal. More recently, however, evidence has been presented that induction of native c-di-GMP phosphodiesterases does not lead to dispersal of P. aeruginosa biofilms. The latter result may discourage attempts to use c-di-GMP signaling as a target for development of anti-biofilm drugs. However, here we demonstrate that induction of the P. aeruginosa c-di-GMP phosphodiesterases PA2133 and BifA indeed does result in dispersal of P. aeruginosa biofilms in both a microtiter tray biofilm assay and in a flow-cell biofilm system.Funding Information
- Natur og Univers, Det Frie Forskningsråd
This publication has 40 references indexed in Scilit:
- Tolerance and Resistance of Pseudomonas aeruginosa Biofilms to Antimicrobial Agents—How P. aeruginosa Can Escape AntibioticsFrontiers in Microbiology, 2019
- Molecular mechanisms of biofilm-based antibiotic resistance and tolerance in pathogenic bacteriaFEMS Microbiology Reviews, 2017
- Cyclic di-GMP: second messenger extraordinaireNature Reviews Microbiology, 2017
- Biofilm DevelopmentMicrobiology Spectrum, 2015
- Pseudomonas aeruginosa biofilm infections: From molecular biofilm biology to new treatment possibilitiesAPMIS, 2014
- Regulation of biofilm formation in Pseudomonas and Burkholderia speciesEnvironmental Microbiology, 2014
- Clearance of Pseudomonas aeruginosa Foreign-Body Biofilm Infections through Reduction of the Cyclic Di-GMP Level in the BacteriaInfection and Immunity, 2013
- Characterization of starvation‐induced dispersion in Pseudomonas putida biofilms: genetic elements and molecular mechanismsMolecular Microbiology, 2010
- Proteins with GGDEF and EAL domains regulatePseudomonas putidabiofilm formation and dispersalFEMS Microbiology Letters, 2006
- Bacterial Biofilms: A Common Cause of Persistent InfectionsScience, 1999