Cardiac endurance training alters plasma profiles of circular RNA MBOAT2
- 1 July 2020
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 319 (1), H13-H21
- https://doi.org/10.1152/ajpheart.00067.2020
Abstract
Marathon running is an extreme physical activity which determines cardiopulmonary adaption of athletes. Circular RNAs (circRNAs) as potential biomarkers in the blood stream have so far not been tested after such strenuous activities. In silico approaches were performed to identify the potential candidate circRNA MBOAT2. Next, we demonstrated high stability and conservation of circRNA MBOAT2 as well as its abundancy in human plasma. In addition to Sanger sequencing of the circRNA specific head-to-tail junction, or back-splice site, we established a synthetic plasmid standard which allowed exact copy number calculations of circRNA MBOAT2. We then analysed plasmatic circRNA MBOAT2 and observed a significantly lower level 24 h after the marathon. Such alterations were correlated to physical exercise parameters confirming the role of circRNA MBOAT2 as a promising non-coding RNA biomarker detecting cardiopulmonary adaption.Keywords
Funding Information
- ERC Consolidator Grant LONGHEART
- DFG Non-coding RNA in the cardiovascular system
This publication has 40 references indexed in Scilit:
- Cell-Type Specific Features of Circular RNA ExpressionPLoS Genetics, 2013
- Circular RNAs are abundant, conserved, and associated with ALU repeatsRNA, 2012
- Myonectin (CTRP15), a Novel Myokine That Links Skeletal Muscle to Systemic Lipid HomeostasisPublished by Elsevier BV ,2012
- Predicting protein associations with long noncoding RNAsNature Methods, 2011
- Lysophospholipid Acyltransferases and Arachidonate Recycling in Human NeutrophilsOnline Journal of Public Health Informatics, 2008
- Recommendations for Chamber Quantification: A Report from the American Society of Echocardiography’s Guidelines and Standards Committee and the Chamber Quantification Writing Group, Developed in Conjunction with the European Association of Echocardiography, a Branch of the European Society of CardiologyJournal of the American Society of Echocardiography, 2005
- Exercise Capacity and Mortality among Men Referred for Exercise TestingThe New England Journal of Medicine, 2002
- Increased Cardiac Sympathetic Activity and Insulin-Like Growth Factor-I Formation Are Associated With Physiological Hypertrophy in AthletesCirculation Research, 2001
- The influence of endurance training on insulin-like growth factor-1 in older individualsMetabolism, 1994
- Physical Fitness as a Predictor of Cardiovascular Mortality in Asymptomatic North American MenThe New England Journal of Medicine, 1988