Identification and Allelic Variants Associated With Cold Tolerance of PmPIAS in Pinctada fucata martensii
Open Access
- 2 March 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Physiology
Abstract
The protein inhibitor of activated STAT (PIAS) functions in diverse aspects, including immune response, cell apoptosis, cell differentiation, and proliferation. In the present study, thePIASin the pearl oysterPinctada fucata martensiiwas characterized. The sequence features of PmPIAS were similar to that of other PIAS sequences with PIAS typical domains, including SAP, Pro-Ile-Asn-Ile-Thr (PINIT), RLD domain, AD, and S/T-rich region. Homologous analysis showed that PmPIAS protein sequence showed the conserved primary structure compared with other species. Ribbon representation of PIAS protein sequences also showed a conserved structure among species, and the PINIT domain and RLD domain showed the conserved structure compared with the sequence ofHomo sapiens. The expression pattern ofPmPIASin different tissues showed significant high expression in the gonad.PmPIASalso exhibited a significantly higher expression in the 1 and 2 days after cold tolerance stress (17°C) and showed its potential in the cold tolerance. The SNP analysis of the exon region ofPmPIASobtained 18 SNPs, and among them, 11 SNPs showed significance among different genotypes and alleles between cold tolerance selection line and base stock, which showed their potential in the breeding for cold tolerance traits.Keywords
This publication has 45 references indexed in Scilit:
- Regulation of early Xenopus development by the PIAS genesDevelopmental Dynamics, 2011
- Characterization of the shsp genes in Drosophila buzzatii and association between the frequency of Valine mutations in hsp23 and climatic variables along a longitudinal gradient in AustraliaCell Stress and Chaperones, 2010
- Gilthead sea bream liver proteome altered at low temperatures by oxidative stressProteomics, 2010
- Selection for Fast Growth in the Chinese Pearl Oyster, Pinctada martensii: Response of the First Generation LineJournal of the World Aquaculture Society, 2009
- Solution structures and DNA binding properties of the N‐terminal SAP domains of SUMO E3 ligases from Saccharomyces cerevisiae and Oryza sativaProteins-Structure Function and Bioinformatics, 2008
- Using Fluorous Amino Acids To Probe the Effects of Changing Hydrophobicity on the Physical and Biological Properties of the β-Hairpin Antimicrobial Peptide Protegrin-1Biochemistry, 2008
- Regulation of gene-activation pathways by PIAS proteins in the immune systemNature Reviews Immunology, 2005
- Regulation of JAK–STAT signalling in the immune systemNature Reviews Immunology, 2003
- The ‘PINIT’ motif, of a newly identified conserved domain of the PIAS protein family, is essential for nuclear retention of PIAS3LFEBS Letters, 2003
- Cold tolerance of three strains of Nile tilapia, Oreochromis niloticus, in ChinaAquaculture, 2002