18F-fluorodeoxyglucose positron emission tomography–computed tomography for assessing organ distribution of stressed red blood cells in mice
Open Access
- 28 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-9
- https://doi.org/10.1038/s41598-021-82100-y
Abstract
Red blood cells (RBCs) stressed by high temperature are similar to senescent or damaged RBCs in pathological conditions. RBCs can be efficiently labelled with 18F-fluorodeoxyglucose (FDG). The aim of this study was to assess stressed RBCs erythrophagocytosis and organ distribution in vivo with the application of 18F-FDG PET/CT. RBCs were induced under high temperature (48 °C) to prepare stressed RBCs. Fluorescence-activated cell sorting (FACS) was used to analyse reactive oxygen species (ROS) generation, intracellular Ca2+ concentration and membrane phosphatidylserine (PS) externalization of RBCs. 18F-FDG was used to label RBCs and assess the erythrophagocytosis. Finally, 18F-FDG PET/CT was applied to reveal and measure the organ distribution of stressed RBCs in mice. Compared with untreated RBCs, stressed RBCs decreased in cell volume and increased in ROS level, intracellular Ca2+ concentration, and PS exposure. RBCs could be labelled by 18F-FDG. Stressed RBCs tended to be phagocytosed by macrophages via assessment of FACS and radioactivity. 18F-FDG PET/CT imaging showed that stressed RBCs were mainly trapped in spleen, while untreated RBCs remained in circulation system. Thus, stressed RBCs can be effectively labelled by 18F-FDG and tend to be trapped in spleen of mice as assessed by PET/CT.Funding Information
- The National Natural Science Foundation of China (81873574)
This publication has 36 references indexed in Scilit:
- Red Blood Cell Survival in Long-term Dialysis PatientsAmerican Journal of Kidney Diseases, 2011
- Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liverBlood, 2011
- Janus Kinase 3 is Expressed in Erythrocytes, Phosphorylated Upon Energy Depletion and Involved in the Regulation of Suicidal Erythrocyte DeathCellular Physiology and Biochemistry, 2011
- Procoagulant activity of long‐term stored red blood cells due to phosphatidylserine exposureTransfusion Medicine, 2010
- Transfusion of red blood cells after prolonged storage produces harmful effects that are mediated by iron and inflammationBlood, 2010
- Temperature sensitivity of suicidal erythrocyte deathEuropean Journal of Clinical Investigation, 2010
- CD47 Is Upregulated on Circulating Hematopoietic Stem Cells and Leukemia Cells to Avoid PhagocytosisCell, 2009
- Initial and subsequent approach for the synthesis of 18FDGSeminars in Nuclear Medicine, 2002
- Monocyte‐mediated erythrocyte destructionTransfusion, 1981
- Quantitative influence of antibody and complement coating of red cells on monocyte-mediated cell lysis.JCI Insight, 1978